Now! 20% off on these prostaglandin ELISA kits:
Prostaglandin E2 ELISA Kit (#514010)
For quantification of PGE2 in plasma, urine, and culture media samples
Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo, PGE2 is rapidly converted to an inactive metabolite (13,14-dihydro-15-keto PGE2) by the prostaglandin 15-dehydrogenase pathway. The half-life of PGE2 in the circulatory system is
approximately 30 seconds and normal plasma levels are 3-12 pg/ml. Cayman Chemical`s PGE2 ELISA has been validated for use with urine, plasma, and culture media samples. In general, urine and culture media samples can be diluted, if necessary, and added directly to the assay well. Plasma samples should be purified prior to use. Because of the rapid metabolism of PGE2, the determination of in vivo PGE2 biosynthesis is often best accomplished by the measurement of PGE2 metabolites. Our Prostaglandin E Metabolite assay (Item No. 514531) converts all major PGE2 metabolites into a single stable derivative which is easily measurable by ELISA.
Prostaglandin E2 Express ELISA Kit (#500141)
For the rapid measurement of PGE2 from biological samples
Cayman Chemical’s PGE2 Express ELISA is a competitive assay that permits the rapid measurement of PGE2 from biological samples, requiring only 1 hour incubation and development times for each step. The foundation of the assay is Cayman’s high-affinity, well-characterized PGE2 monoclonal, the same used in our PGE2 Monoclonal ELISA kit (Item No. 514010). While Item No. 514010 offers superior sensitivity (80% B/B0 ~15 pg/ml), the new PGE2 Express Assay offers the convenience of a fast assay while still achieving an 80% B/B0 of 36 pg/ml and an assay range of 15.6-2,000 pg/ml.
Prostaglandin E Metabolite ELISA Kit (#514531)
Prostaglandin E2 (PGE2) is produced by a variety of cell types which, in general, do not contain the enzymes required for metabolism of PGE2. Thus, cultured endothelial cells or osteoblasts will release PGE2 into the culture medium, where it will accumulate without appreciable metabolism. The direct assay of PGE2 from the medium is a good way to measure PGE2 production from these cells. PGE2 is rapidly converted in vivo to its 13,14-dihydro-15-keto metabolite, with more than 90% of circulating PGE2 cleared by a single passage through the lungs. Unfortunately, this metabolite is not chemically stable and undergoes a variable amount of
degradation to PGA products. For this reason, blood, urine, or other samples from whole animals or humans often contain very little intact PGE2, and measurement of the metabolites is necessary to provide a reliable estimate of actual PGE2 production. Cayman’s PGE Metabolite assay was developed as a method of converting 13,14-dihydro-15-keto PGA2 and 13,14-dihydro-15-keto PGE2 to a single, stable derivative that could be easily quantified by ELISA. This assay is therefore the method of choice if the samples in question have undergone extensive metabolism prior to collection.