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Next Generation Sequencing (NGS)

Our products for Next Generation Sequencing includes sample preparation, automated size selection and analysis software.


NEBNext®

NEBNext®

NEBNext® reagents from New England BioLabs (NEB) are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mix sets and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value.

Each set of reagents is functionally validated together through construction of a library from an accepted DNA or RNA reference sample that is then sequenced on the appropriate platform. Whenever a new lot of any of the reagents is introduced into a set, the set is re-validated by library construction and sequencing.

NEBNext reagents are available for sample preparation for Illumina®, 454™, SOLiD™ and Ion Torrent™ sequencing platforms in multiple formats, including reagents sets, master mix sets and modules.

DNA/RNA Size Selection: Pippin

DNA/RNA Size Selection: Pippin

The Pippin-family facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer with no possibility of cross contamination.

Pippin’s disposable gel cassettes are precast with agarose. DNA sizing is determined by the detection of markers in a dedicated lane or internally in the sample lane and using those to select and collect the desired fragments based on user-input values. Each sample lane is physically separate to eliminate the possibility of sample cross-contamination and features a branched configuration with three electrodes. DNA separates along a gel column until the programmed fragment range reaches the branch point. The instrument then switches the active electrode to divert DNA into a membrane-bound buffer chamber. When the size range is collected, the active electrode is switched back to the separation channel. The desired sample are removed with a standard pipette.

DNA sizing is available with the
Pippin Prep (for fragments from 90 bp to 1.5 Kb) and with the BluePippin (a pulsed-field version for fragments from 50 bp to 50 Kb). Also available as High Throughout - PippinHT

Sequencher DNA Sequence Analysis Software

Sequencher DNA Sequence Analysis Software

Sequencher brings some of the best peer-reviewed NGS algorithms out of the command line and into your hands. Beginners can start aligning their NGS data immediately while advanced users still have access to each command line input via an easy to understand interface. Perform SNP analyses, align methylated data, and perform de novo assemblies or reference-guided alignments.

Sequencher's latest NGS functionality includes RNA-Seq data analysis with the addition of the Cufflinks suite. Use your NGS data to explore differential gene expression with multiple graphs to display your results.

 
 
 

BioNordika AS ♦ Gladengveien 3 B, 0661 Oslo ♦ Tel: 23 03 58 00 ♦ Faks: 23 03 58 01 ♦ E-post: info@bionordika.no


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