Aktiviteter

Frokostseminar: Western blotting, 23 okt. 2019, Oslo

Dato: 23. oktober, 2019
Tid: 8.30 - 9.45
Sted: BioNordika, Gladengveien 3B, 4 etg

Solutions for successful Western Blotting

Abstrakt: 
Western blotting, also called immunoblotting, is a widely used and accepted technique to detect levels of protein expression in a cell or tissue extract. The precise binding that occurs between an antibody and its target protein
epitope allows detection of highly specific amino acid sequences within a protein.
During this talk, we will highlight the critical steps in the western blot protocol and demonstrate how protocol changes can affect the final outcome of your blot. We’ll also provide recommendations on reagents and procedures based on our extensive experience with western blotting as part of our validation, lot testing, and technical support process. We will also discuss the importance of using a well-validated antibody in your western blot experiments.

Foredragsholder: 
Federica Cioffi, PhD, Field Application Scientist, Cell Signaling Technology

Seminaret er gratis, inkluderer enkel frokostservering, og det vil bli holdt på engelsk.

Registrering: 
Send din kontaktinformasjon til malfrid@bionordika.no 
Påmelding senest 15. oktober. 

Program

Frokostseminar: Western blotting, 23 okt. 2019, Oslo

CyTOF/Hyperion seminar, 23. okt. 2019, Oslo

Dato: 23. oktober, 2019
Tid: 13.00 - 14.00
Sted: Møterom, 4. etg, Forskningsbygget, Radiumhospitalet

The use of highly validated recombinant monoclonal antibodies in cell suspension and imaging mass cytometry platforms

Abstrakt: 
Mass cytometry uses metal-tagged antibodies for multiplex analysis of protein markers in single cells (cell suspension CyTOF) and in tissue sections (imaging CyTOF). The former requires flow cytometry validated antibodies and the latter requires IHC validated antibodies. While antibodies have been a key reagent driving advancements in biomedical research, there are increasing numbers of publications raising concerns about the quality of antibodies and their irreproducibility.

In this seminar, I will discuss how Cell Signaling Technology’s antibody validation efforts can lead to high quality rabbit monoclonal antibodies showing exquisite specificity, sensitivity, and reproducibility across multiple applications, including IHC and flow cytometry. The recombinant nature of our monoclonal antibodies assures consistent performance and availability making them ideal for inclusion in CyTOF panels. We will also discuss the protein-free formulations and high concentration of antibodies required for metal conjugation, and the custom solutions possible from Cell Signaling Technology

Foredragsholder: 
Sriram Aravamudhan, PhD, Proteomics Product Manager Europe, Cell Signaling Technology

Seminaret er gratis, og det vil bli holdt på engelsk.

Registrering: 
Send din kontaktinformasjon til malfrid@bionordika.no 
Påmelding senest 15. oktober. 

Program

CyTOF/Hyperion seminar, 23. okt. 2019, Oslo

CyTOF/Hyperion seminar, 22. okt. 2019, Bergen

Dato: 22. oktober, 2019
Tid: 13.00 - 14.00
Sted: Rom 5.1-5.2, Lab-bygget, 5. etg

The use of highly validated recombinant monoclonal antibodies in cell suspension and imaging mass cytometry platforms

Abstrakt: 
Mass cytometry uses metal-tagged antibodies for multiplex analysis of protein markers in single cells (cell suspension CyTOF) and in tissue sections (imaging CyTOF). The former requires flow cytometry validated antibodies and the latter requires IHC validated antibodies. While antibodies have been a key reagent driving advancements in biomedical research, there are increasing numbers of publications raising concerns about the quality of antibodies and their irreproducibility.

In this seminar, I will discuss how Cell Signaling Technology’s antibody validation efforts can lead to high quality rabbit monoclonal antibodies showing exquisite specificity, sensitivity, and reproducibility across multiple applications, including IHC and flow cytometry. The recombinant nature of our monoclonal antibodies assures consistent performance and availability making them ideal for inclusion in CyTOF panels. We will also discuss the protein-free formulations and high concentration of antibodies required for metal conjugation, and the custom solutions possible from Cell Signaling Technology

Foredragsholder: 
Sriram Aravamudhan, PhD, Proteomics Product Manager Europe, Cell Signaling Technology

Seminaret er gratis, og det vil bli holdt på engelsk.

Registrering: 
Send din kontaktinformasjon til malfrid@bionordika.no 
Påmelding senest 15. oktober. 

Program

CyTOF/Hyperion seminar, 22. okt. 2019, Bergen