PCR & qPCR (amplification)
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Knowledge and insights about PCR & qPCR (amplification) from the Science Hub
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23.01.2024
Having trouble when amplifying GC-rich sequences? Here are four tips on how to troubleshoot
GC-rich templates in DNA sequences have 60% or more G (guanine) or C (cytosine) bases. Amplifying GC-rich templates may require adjusting reagents, annealing temperature, and using specialized master mixes or standalone polymerases for optimal results, and in this blog article you get four tips on how to troubleshot.
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23.01.2024
How to evaluate your qPCR and RT-qPCR results
qPCR and RT-qPCR methods are described, their usefulness and power as well as their sensitivity and vulnerability. In addition, it is explained how qPCR evaluation is accomplished by applying the “MIQE” guidelines and NEB´s “dots in boxes” quality scoring methods.
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