News

Request your personal copy of the 2023/2024 version of the NEB catalog together with the updated Restriction Enzyme Poster. The must-have for every molecular biology lab.

Discover the most up-to-date details on NEB products, along with expert recommendations to identify the perfect fit for your specific applications.

Don't miss the widely popular technical reference section, brimming with valuable insights on optimizing product performance, technical tips, troubleshooting guides, and a wealth of other indispensable information.

Read about how researchers at NEB are working to improve their understanding of the genome, epigenome and transcriptome and much more!

Download here.

• Feature Article: Monitoring trick and mosquito infections using LAMP: Screening for EEE and beyond
• (m)RNA capping
• Q5 DNA Polymerase – the finest in PCR fidelity for over 10 years
• General Guidelines for PCR Optimization
• NGS Library Prep – Spot on NEBNext Ultra II RNA Library Prep Kits

Download here

With Q5 Blood Direct, you can run PCR directly on blood without DNA extraction.

The master mix is compatible with samples containing up to 30% whole blood/EDTA blood. It can also be used on dried blood spots (DBS) on filter paper from Whatman^® 903, FTA Elute or FTA Classic and does not require pre-treatment to remove inhibitors. The mix uses Q5 polymerase, one of our best-selling polymerases, and is well suited for DNA sequences up to 7.5 kb. The mix can also be used on GC rich sequences with between 23 - 77% GC.

Learn more.

Check out all our Q5 DNA polymerase products here. 

NextOmics/GrandOmics is the largest third-generation sequencing service company in China. The NextOmics division focuses on animal, plant and bacterial genome sequencing, while GrandOmics focuses on human genome sequencing.

Recently, NextOmics/GrandOmics has disclosed some examples of its PromethIon output. Typical size distributions from the PromethIon flow cell show read N50 values as high as 140kb, with an impressive 28% of the reads longer than 200kb.

For ultra-long reads, size selection is performed using the Sage HLS instrument.

Read more here. 

The µChIPmentation kit for histones from Diagneode is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.

The number of sample transfers from tube to tube have been limited to reduce loss of DNA. Thus only 3 tubes per sample are used in the workflow (from cell fixation to library prurification).

Reduced number of steps, reduced number of sample transfer and ChIPmentation technology itself enable for efficient and robust ChIP-seq on limited amount of sample.

Vectashield Plus Antifade Mounting Media, a non-setting antifade formulation. This formulation has improved the range of compatible fluorophores and eliminated blue background. 

Why VECTASHIELD PLUS?
• No inherent toning or background
• Superior signal retention from the visible to far red spectrum
• Sections can be viewed in 1 hour after mounting
• Available with or without DAPI counterstain
• Superior non-setting formulation
• Available in two convenient sizes - 2 ml or 10 ml
• Compatible with commonly used fluorophores

Learn more

EdgeBio offers a large selection of products for Sanger sequencing:
• Dyes
• Purification plates
• Magnetic beads

Check out their products in this catalog:
"Make your Sanger sequencing cost effective". 

Download the pdf. 

The health of cells in culture is critical to the success of your experiments. Have you ever been excited about the experimental results of a knock-down, drug treatment, or culture condition, only to realize later that the effects are skewed due to the amount of cell death that occurred in your samples? Measuring and comparing cell viability in your assays is important, whether it’s the data you’re pursuing or an important control in your experiment. 

Read more in this blog post by Tamar A., Cell Signaling Technology. 

Maximize your protein production

eCHO medium from Lonza is designed to maximize protein integrity, positively affected by low levels of lactate and late-stage viability. With over 40 years of expertise in media development, Lonza is proud to introduce eCHO Basal Medium and Feed. The serum-free, chemically defined, hydrolysate-free and non-animal origin (NAO) eCHO Medium optimizes your cell proliferation and protein production.

Ferroptosis was recently identified as a form of programmed cell death. Activation of ferroptosis results in destruction of certain cancer cells, whereas inhibition of this process may protect organisms from neurodegeneration

CaymanCurrents no.4, 2018 is focused on ferroptosis and there you will find amongst other:  
• The Ferroptosis Pathway: Structure, Function, and Modulation
• Ferroptosis Inducers
• Ferroptosis Inhibitors

Download the pdf here.

Check out the VECTASTAIN^® Elite^® ABC Universal PLUS Kit (#PK-8200) and three new ImmPRESS^® Universal PLUS Polymer Kit (#MP-7800, #MP-7801 og #MP-7802) from Vector Laboratories.

These peroxidase (HRP) IHC kits contain matching volume reagents, provided as prediluted, ready-to-use formats, and importantly, also include our endogenous enzyme quenching solution BLOXALL^® (SP-6000) and the ImmPACT^® DAB EqV peroxidase substrate (SK-4103).

These “PLUS” kits are suitable for both manual and automated IHC staining applications, and they are intended to provide a more streamlined and convenient workflow.

NEBNext^® is now available for single cell and ultra-low RNA inputs!

NEBNext^® Single Cell/Low Input RNA Library Prep Kit for Illumina^®
This unique workflow meets the demand for a highly sensitive, yet robust method that consistently enables generation of high quality sequencing data from single cell or ultra-low input RNA.

• Generate the highest yields of high-quality sequencing libraries from single cells, or as little as 2 pg - 200 ng RNA
• Experience unmatched detection of low abundance transcripts
• Rely on consistent transcript detection for a wide range of input amounts and sample types
• Observe uniform transcript coverage, regardless of input amount or sample type
• Use with a variety of RNA inputs, including cultured or primary cells, or total RNA
• Save time with a fast, streamlined workflow, minimal handling steps and hands-on time

Technical note

The new Monarch Total RNA Miniprep Kit from NEB is optimized for use with a wide variety of sample types, including cells, tissue, blood and more, so that one convenient kit can serve your RNA purification needs.

Learn more about the nucleic acid purification kits from NEB here.