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µChIPmentation kit for histones

The µChIPmentation kit for histones from Diagneode is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.

The number of sample transfers from tube to tube have been limited to reduce loss of DNA. Thus only 3 tubes per sample are used in the workflow (from cell fixation to library prurification).

Reduced number of steps, reduced number of sample transfer and ChIPmentation technology itself enable for efficient and robust ChIP-seq on limited amount of sample.

µChIPmentation kit for histones

All reagents needed for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation is included in the kit, whereas the primer indexes for multiplexing are not included in the kit and have to be purchase separately. 

C01011012µChIPmentation Kit for Histones, 8 reactions
C01011011: µChIPmentation Kit for Histones, 24 reactions

Download the manual

Vectashield Plus Antifade Mounting Media

Vectashield Plus Antifade Mounting Media, a non-setting antifade formulation. This formulation has improved the range of compatible fluorophores and eliminated blue background. 

Why VECTASHIELD PLUS?
• No inherent toning or background
• Superior signal retention from the visible to far red spectrum
• Sections can be viewed in 1 hour after mounting
• Available with or without DAPI counterstain
• Superior non-setting formulation
• Available in two convenient sizes - 2 ml or 10 ml
• Compatible with commonly used fluorophores

Learn more

Vectashield Plus Antifade Mounting Media
Cat. no.  Product Amount
H-2000-2 VECTASHIELD® PLUS Antifade Mounting Medium with DAPI 2 ml
H-2000-10 VECTASHIELD® PLUS Antifade Mounting Medium with DAPI 10 ml
H-1900-2 VECTASHIELD® PLUS Antifade Mounting Medium 2 ml
H-1900-10 VECTASHIELD® PLUS Antifade Mounting Medium 10 ml
...  Learn more   

All you need for your Sanger sequencing

EdgeBio offers a large selection of products for Sanger sequencing:
• Dyes
• Purification plates
• Magnetic beads

Check out their products in this catalog:
"Make your Sanger sequencing cost effective". 

Download the pdf

All you need for your Sanger sequencing

Cell Process: What is cell viability and how can it be measured?

The health of cells in culture is critical to the success of your experiments. Have you ever been excited about the experimental results of a knock-down, drug treatment, or culture condition, only to realize later that the effects are skewed due to the amount of cell death that occurred in your samples? Measuring and comparing cell viability in your assays is important, whether it’s the data you’re pursuing or an important control in your experiment. 

Read more in this blog post by Tamar A., Cell Signaling Technology. 

Cell Process: What is cell viability and how can it be measured?

Mya 4 - one chemistry workstation for your reaction optimisation needs

Maximum flexibility in a single piece of equipment from 2 ml to 400 ml.

Do you routinely perform
Test reactions in vials and tubes?
Optimisation work in small flasks or reactors?

What if you could do it all using a single piece of kit?
Then Mya 4 reaction station is the solution for you.

Product brochure
Demo video

Mya 4 - one chemistry workstation for your reaction optimisation needs

Radleys Catalog 2020

Check out the new catalog from Radleys with their high quality equipment for chemical synthesis, process development, evaporation and work-up. 

Download the catalog

Radleys Catalog 2020

eCHO Medium

Maximize your protein production

eCHO medium from Lonza is designed to maximize protein integrity, positively affected by low levels of lactate and late-stage viability. With over 40 years of expertise in media development, Lonza is proud to introduce eCHO Basal Medium and Feed. The serum-free, chemically defined, hydrolysate-free and non-animal origin (NAO) eCHO Medium optimizes your cell proliferation and protein production.

eCHO Medium

Improved productivity:
Consistent titer levels of 3.0 g/L using a qualified clone within 14 culture days in a fed batch culture.

Ease of use:
One part liquid and one part feed in both powder and liquid formats

Consistency:
Liquid products produced from (one) powder.

Product Quality:
High viability to end of cell culture in addition to low lactate levels, securing protein integrity and posttranslational modification.

Learn more

Ferroptose - a new form of cell death

Ferroptosis was recently identified as a form of programmed cell death. Activation of ferroptosis results in destruction of certain cancer cells, whereas inhibition of this process may protect organisms from neurodegeneration

CaymanCurrents no.4, 2018 is focused on ferroptosis and there you will find amongst other:  
• The Ferroptosis Pathway: Structure, Function, and Modulation
• Ferroptosis Inducers
• Ferroptosis Inhibitors

Download the pdf here.

Ferroptose - a new form of cell death

New VECTASTAIN® ABC and ImmPRESS® Polymer IHC kits

We are pleased to introduce the VECTASTAIN® Elite® ABC Universal PLUS Kit (#PK-8200) and three new ImmPRESS® Universal PLUS Polymer Kit (#MP-7800, #MP-7801 og #MP-7802) from Vector Laboratories.

These new peroxidase (HRP) IHC kits contain matching volume reagents, provided as prediluted, ready-to-use formats, and importantly, also include our endogenous enzyme quenching solution BLOXALL® (SP-6000) and the ImmPACT® DAB EqV peroxidase substrate (SK-4103).

These “PLUS” kits are suitable for both manual and automated IHC staining applications, and they are intended to provide a more streamlined and convenient workflow.

New VECTASTAIN® ABC and ImmPRESS® Polymer IHC kits

NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®

NEBNext® is now available for single cell and ultra-low RNA inputs!

NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®
This unique workflow meets the demand for a highly sensitive, yet robust method that consistently enables generation of high quality sequencing data from single cell or ultra-low input RNA.

• Generate the highest yields of high-quality sequencing libraries from single cells, or as little as 2 pg - 200 ng RNA
• Experience unmatched detection of low abundance transcripts
• Rely on consistent transcript detection for a wide range of input amounts and sample types
• Observe uniform transcript coverage, regardless of input amount or sample type
• Use with a variety of RNA inputs, including cultured or primary cells, or total RNA
• Save time with a fast, streamlined workflow, minimal handling steps and hands-on time

Technical note

NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®

Monarch Total RNA MiniPrep kit

The new Monarch Total RNA Miniprep Kit from NEB is optimized for use with a wide variety of sample types, including cells, tissue, blood and more, so that one convenient kit can serve your RNA purification needs.

Learn more about the nucleic acid purification kits from NEB here.

Monarch Total RNA MiniPrep kit