The µChIPmentation kit for histones from Diagneode is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries.
The number of sample transfers from tube to tube have been limited to reduce loss of DNA. Thus only 3 tubes per sample are used in the workflow (from cell fixation to library prurification).
Reduced number of steps, reduced number of sample transfer and ChIPmentation technology itself enable for efficient and robust ChIP-seq on limited amount of sample.
All reagents needed for chromatin preparation, chromatin immunoprecipitation and library preparation using tagmentation is included in the kit, whereas the primer indexes for multiplexing are not included in the kit and have to be purchase separately.
C01011012: µChIPmentation Kit for Histones, 8 reactions
C01011011: µChIPmentation Kit for Histones, 24 reactions