Sample prep for ultra-long Nanopore Sequencing

NextOmics/GrandOmics is the largest third-generation sequencing service company in China. The NextOmics division focuses on animal, plant and bacterial genome sequencing, while GrandOmics focuses on human genome sequencing.

Recently, NextOmics/GrandOmics has disclosed some examples of its PromethIon output. Typical size distributions from the PromethIon flow cell show read N50 values as high as 140kb, with an impressive 28% of the reads longer than 200kb.

For ultra-long reads, size selection is performed using the Sage HLS instrument.

Read more here.