Cloning competent cell strains from NEB

Choose the right cells for your cloning applications from our high efficiency competent cell strains from New England Biolabs.

There are many properties to consider when choosing a strain for your experiment, and requirements such as plasmid preparation, blue/white screening, cytoplasmic disulfide bond formation, and fast colony growth matter.

Cloning competent cell strains

Things to consider when you choose your competent cells:

  • If you need tight expression control, choose a strain with lacIq mutation
  • If you are using Gateway vectors, you will be happy to know that many NEB competent E.coli do a great job battling with ccdB toxin, but you can also pick up the ccdB sensitive ones for your use
  • If you want to do traditional blue-white screening, almost all the NEB E. coli strains are lacZ deletion mutants
  • If your plasmid has long repeat regions (for instance lentiviral vectors), you may want to have recA deficient E.coli strain from NEB 
  • If you are still left pondering the best option, purchase NEB Cloning Competent E. coli sampler, see the table below and contact us for any questions

Advantages with our E. coli competent cell strains:

  • High transformation efficiencies
  • Free of animal products
  • T1 phage resistance (fhuA2)
  • All cloning strains and most expression strains are endA deficient 
  • Most strains include media and control plasmid
  • Compatible with NEBuilder HiFi DNA Assembly and Gibson Assembly reactions, as well as ligation reactions. No dilution required!
  • Strains also available for cloning of toxic genes
  • Outgrowth medium and control plasmid are included
  • A variety of convenient formats, including single use transformation tubes and, on request, 96 well plates

20% off on all competent cells from NEB!

 

Cat. no. Product Characteristics
C2984 NEB Turbo Competent E. coli (High Efficiency) • Fastest growth – colonies visible after 6.5 hours
• Plasmid preparation after 4 hours
C2987 NEB 5-alpha Competent E. coli (High Efficiency) • Versatile cloning strain
• DH5α™ derivative
C2992 NEB 5-alpha F´ Iq Competent E. coli (High
Efficiency)		 • Toxic gene cloning
• F´ strain with extremely high transformation efficiency
C3019 NEB 10-beta Competent E. coli (High Efficiency) • Large plasmid and BAC cloning
• DH10B™ derivative
C2925 dam-/dcm- Competent E. coli • Dam/Dcm methyltransferase free plasmid growth
C3040 NEB Stable Competent E. coli • High quality plasmid preparations due to endA mutation
• Rapid growth recA strain
• T1 phage resistance (fhuA)
• Free of animal products
C1010S NEB Cloning Competent E. coli Sampler • Contains NEB 5-alpha, NEB 10-beta, NEB Stable, NEB turbo
The offer is valid until 31.12.2023. Promotional code #BNH2-23 must be referenced when ordering to receive the discount.