Golden Gate Assembly

Golden Gate Assembly from NEB, for fast and reliable DNA Cloning of multiple fragments!

To simplify the workflow of assembling 2 or more than 50 fragments into the vector, NEB offer two kits for Golden Gate Assembly featuring BsaI-HFv2 or BsmBI-v2. In a single step, Golden Gate Assembly utilizes simultaneous digestion with Type IIS restriction enzymes and ligation by a DNA ligase to enable the seamless assembly of multiple fragments. 

Golden Gate Assembly

Choose from over 45 types of IIS restriction enzymes to drive the assembly by recognizing asymmetric DNA sequences and cleaving outside their recognition sequence.

Golden Gate Assembly advantages:

  • Seamless & ordered cloning leaving no assembly scars
  • Assemble multiple fragments (2 to 50+) in a single reaction
  • Save time with 5 minutes single insert cloning
  • High efficiency, even with GC rich regions and areas of repeats
  • Generate libraries with high efficiencies
  • Use fragment sizes from less than 100 bp to over 15 kb
Cat. no. Product Size Special price
E1602S NEB Golden Gate Assembly kit (BsmBI-v2) 20 reactions 1 851,-
E1602L NEB Golden Gate Assembly kit (BsmBI-v2) 100 reactions 5 257,-
E1601S NEB Golden Gate Assembly kit (BsaI-HF-v2) 20 reactions 1 851,-
E1601L NEB Golden Gate Assembly kit (BsaI-HF-v2) 100 reactions 5 257,-
The offers are listed excl. VAT and shipping charges, and is valid until 31.12.2021. Promotional code #BN3-21 must be referenced when ordering to receive the special prices.