NEBuilder® HiFi DNA assembly cloning kit

NEBuilder was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotides or different sizes of DNA fragments with varied overlaps (15–30 bp). It has utility for the synthetic biology community, as well as those interested in one-step cloning of multiple fragments due to its ease of use, flexibility and simple master-mix format.

NEBuilder® HiFi DNA assembly cloning kit
  • Assembly and transformation in just under two hours
  • Enjoy less screening/re-sequencing of constructs, with virtually error-free, high-fidelity assembly
  • Flexible sequence design (scar-less cloning)
  • No PCR clean-up step required
  • Join DNA fragments together more efficiently, even with larger fragments or low DNA inputs
  • Use NEBuilder HiFi in successive rounds of assembly, because it removes 5 ́ and 3 ́ end mismatches. (Save time by avoiding time-consuming PCR amplification steps.)
  • Bridge two ds-fragments with a synthetic ss-DNA oligo for simple and fast construction (e.g., linker insertion or gRNA library)
  • Use with lower DNA input requirements
  • Flexible sequence design (scar-less cloning)
  • Easily adapted for multiple DNA manipulations, including site-directed mutagenesis
  • Supplied with 10 x 50 μl vials of NEB® 5-alpha Competent E. coli (High Efficiency) (NEB #C2987)

Kr. 2 125,- 


Cat. no. E5520S: NEBuilder HiFi DNA Assembly Cloning Kit, 10 reactions
The offer is listed excl. VAT and shipping charges. Promotional code #BN2-21 must be referenced when ordering to receive the discount.