Antibodies

We offer primary and secondary antibodies, controls etc. of the best quality from our suppliers: Cayman Chemical, Cell Signaling Technology (CST), GenScript, Origene, Rockland, and Vector Laboratories.
Feel free to contact us if you cannot find what you are looking for, we are happy to assist you.
We also offer antibody production if the antibody you need is not available commercially.


Carrier free antibodies

Antibodies are offered in a carrier free solution from Cell Signaling Technology (CST) to be used in multiplexed single cell experiments and imaging (mass cytometry applications). The antibodies are:

• Validated in relevant applicaions such as flow and/or IHC
• Validated for specificity, sensitivity and reproducibility
• Free of BSA and other carrier proteins that can interfer with heavy metal conjugation
• Available in high concentrations (>1mg/ml), which is minimum requirement in order to conjugate the antibody​​

Carrier free antibodies

When choosing antibodies for MaxPar® metal conjuation in applications such as CyTOF, single cell, Helios™ (Fluidigm) or imaging Hyperion™ (Fluidigm), it is important that the antibodies already are validated in relevant applications. Antibodies from CST are subject to strict validation and specificity testing in order to ensure reproducible results you can trust. The antibody you choose should be matched to your sample preparation method. For FFPE samples you can select from CST`s portfolio of IF-paraffin or IHC-validated antibodies, while for frozen tissue you can consider the IF-Frozen validated antibodies.

Choose antibodies from CST in your mass cytometry experiments to ensure good results!

Do you want an offer for carrier free antibodies, please contact us.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
CyTOF®, Helios, Hyperion, Imaging Mass Cytometry, and MaxPar® are trademarks of Fluidigm, Inc.

ALK and ROS1

Anaplastic lymphoma kinase (ALK) and c-ros oncogene 1 (ROS1) are two proteins of the same family of receptor tyrosine kinases.

Research studies have shown that mutated versions of ALK and ROS1 proteins can function as oncogenic drivers, and that these proteins are expressed in various forms of cancer, as for example non-small cell lung cancer (NSCLC).

Cell Signaling Technology (CST) offer two very sensitive antibodies for detection of ROS1 and ALK proteins. The antibodies are produced by immunizing animals with C-terminal epitopes, which makes it possible to detect both full-length proteins as well as the mutated C-terminal fusion proteins. The antibody sensitivity is so high that you may detect endogenous levels of ROS1 and ALK. The antibodies does not cross-react with other related proteins when analyzed by western blot.


Picture: Immunohistochemical analysis of paraffin-embedded human lung carcinomas showing distinct localization using ROS1 (D4D6®) Rabbit mAb.
ALK and ROS1
ALK and ROS1
ROS1 (D4D6®) Rabbit mAb (#3287)


Reactivity: human
Sensitivity: endogenous
MW (kDa): 258, 110, 50-80
Source/Isotype: rabbit IgG

Application dilutions:
Western blotting: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:250
Immunofluorescence (Immunocytochemistry): 1:200 - 1:800
Flow Cytometry: 1:200 - 1:800

3287S Cat. no. 3287S  Datasheet

In the "Nasjonalt handlingsprogram med retningslinjer for diagnostikk, behandling og oppfølging av lungekreft, mesoteliom og thymom" from Helsedirektoratet it is written:  
"Rearrangering av ROS1-genet forekommer i 1-2 % av adenokarsinom i lunge, og kan påvises ved FISH, RT-PCR eller NGS. Immunhistokjemisk undersøkelse med ett av to aktuelle antistoff (klon D4D6 fra Cell Signaling Technology og klon SP384 fra Ventana Medical Systems), kan brukes som screening."
 

ALK (D5F3®) XP® Rabbit mAb


Reactivity: human
Sensitivity: endogenous
MW (kDa): 220 (ALK), 80 (NPM-ALK), 117 (EML4-ALK v1), 86 (EML4-ALK v3)Source/Isotype: rabbit IgG

Application dilutions:
Western blotting: 1:2000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:250
Flow Cytometry: 1:400

3633S ALK (D5F3®) XP® Rabbit mAb, 100 ul Datasheet
Prices and ordering

Prices are available in our webshop. You may also contact us by e-mail for price information and ordering.

SSX and SS18-SSX

SS18 is a protein that has been shown to be a part of the SWI/SNF complex. The SS18-SSX fusion proteins are a result of in-frame fusions that fuse the SS18 gene on chromosome 18 with X chromosome genes SSX1, SSX2, and to a lesser extent SSX4.

Human synovial sarcoma (SS) accounts for 8 - 10 % of all soft tissue malignancies and 95% of these malignancies express the recurrent translocation of the SS18 gene on chromosome 18.

Cell Signaling Technology (CST) offers an antibody for specific detection of the fusion protein: SS18-SSX (E9X9V) XP® Rabbit mAb (#72364). This antibody recognizes endogenous levels of the SS18-SSX fusion protein. This antibody does not cross-react with wild-type SS18 or SSX proteins.

CST also offer a SSX (E5A2C) Rabbit mAb (Carboxy-terminal Antigen) antibody (#23855) recognizes endogenous levels of total SSX1-4 proteins. This antibody also detects the SS18-SSX fusion protein.

72364S SS18-SSX (E9X9V) XP® Rabbit mAb, 100 ul WB, IP, IHC-Bond, IHC-P, ChIP, ChIP-seq Datasheet
23855S SSX (E5A2C) Rabbit mAb (Carboxy-terminal Antigen), 100 ul WB, IP, IHC-Bond, IHC-P Datasheet
Prices and ordering

Prices are available in our webshop. You may also contact us by e-mail for price information and ordering.

Antibody suppliers

Cayman Chemical

Offers primary and secondary antibodies for various applications, such as western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Their antibodies are validated for biological tissues and cell lysis and are reliable tools for your research. Antibodies to receptors, proteins, enzymes, and post-translational modifications for eicosanoids, neurobiology, immunology, inflammation, cancer and epigenetic research. Secondary antibodies are available with HRP, AP, fluorescein, rhodamine, phycoerythrin, APC, europium, biotin, Texas red and more.

Cell Signaling Technology (CST)

A wide variety of primary and secondary antibodies, many of which have been validated for multiple applications such as Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (F), chromatin immunoprecipitation (ChIP) and sandwich immunoassays (ELISA).

The antibodies are divided into signaling pathways such as e.g. chromatin / epigenetics, MAP kinase signaling, apoptosis & autophagy, PI3K / Akt signaling, translation control & RNA regulation, cell cycle & DNA damage, cellular metabolism, stem cell markers, immunology & inflammation, cytoskeletal regulation, neurobiology, angiogenesis, and adhesion & extracellular matrix.

GenScript

Provides a broad range of high-quality Primary and Secondary antibodies such as Anti-Epitope tag, Immune Checkpoint, Cas 9 and more. 

OriGene

More than 50,000 primary antibodies, including TrueMAB mouse monoclonal and other polyclonal antibodies to human proteins.

Their antibodies include::
• UltraMAB IHC antibodies
• IVD antibodies
• TrueMAB primary antibodies
• Sekundary antibodies like anti-tag antibodies 

Rockland Immunochemicals

Primary antibodies in the categories of apolipoprotein, apoptosis, cancer, cardiovascular, cell biology, cell cycle & checkpoint control, chromatin & nuclear signaling, developmental biology, DNA damage & repair, epigenetics, glucose & energy metabolism, microbiology, neurobiology, phosphospecific, serum protein component, neurobiology, phosphospecific, serum protein component, neurobiology, phosphospecific, serum protein component, signal transduction and phospho-specific, serum protein component, signal transduction, and phospho-specific, serum protein component, signal transduction and stem cell.

They also offer tag-specific antibodies and have a very good selection of secondary antibodies, both in regards to specificity and type of conjugate.

Vector Laboratories

Vector Laboratories specializes in products for immunohistochemistry, immunocytometry and immunofluorescence. This is reflected in their selection of primary, secondary and control antibodies, as well as antibodies to different types of tags and markers.

Antibody production

Are you looking for an antibody, but cannot find it commercially?
We offer custom polyclonal and monoclonal antibody production, and can make a set up according to your needs. 

...  Learn more   
Antibody production

Contact person: Christine Vimo

Christine has a bachelor`s degree in biology and a master`s degree in molecular biology. Contact her if you have any questions about antibodies or the products within the areas western blotting, IHC, IF and flow, and anything you should need for your molecular biology experiments. 

christine@bionordika.no

Contact person: Christine Vimo