Mycoplasma detection

One of the most common contaminants in cell culture laboratories is mycoplasma. Cells are usually infected by cross-contamination from not tested infected cells (eg, through aerosols generated during pipetting, using the same medium bottles, working with more than one cell type at a time), contaminated material, contaminated donor tissue (<1%), or direct infection from the researcher.

Mycoplasma grows slowly and does not necessarily kill the cells, but affects different cellular parameters, such as changing the cell membrane's antigenicity, creating chromosomal level changes, inhibiting cell metabolism and fragmenting DNA.

To ensure accurate and reliable results from cell culture experiments, it is important to regularly test cell cultures for mycoplasma contamination. It can be challenging to detect mycoplasmas contamination because it does not cause change in pH or turbidity of the cell culture medium, which are common indicators of other contaminants.

We recommend MycoAlert ™ Mycoplasma Detection Kit from Lonza, a safe and easy test that takes very little time to perform. 

MycoAlert™ Mycoplasma Detection Kit

The MycoAlert™ Mycoplasma Detection Kit from Lonza, is a selective biochemical test that exploits the activity of mycoplasmal enzymes. These enzymes are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.

• Convenient and fast
• Simply add two reagents to the culture supernatant
• Perform two luminescence readings
• Results in less than 20 min
• Easy interpretation of results
• Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
• Negligible interference with media components

MycoAlert™ Mycoplasma Detection Kit

MycoAlert™ PLUS Mycoplasma Detection Kit generates a higher light output compared to the first-generation kit.
This allows for:
• Use with less sensitive plate luminometers or multifunctional readers
• Testing of unused media, media supplements, or water

The positive control for the MycoAlert™ Mycoplasma Detection Kit is available separately in the MycoAlert™ Assay Control Set (#LT07-518). The control set includes a lyophilized positive control and assay buffer for reconstitution, which also serves as a negative control. We always recommend using the control set when performing assays.

LT07-118 MycoAlert Detection Kit (10 tests) Manual
LT07-218 MycoAlert Detection Kit (25 Tests) Manual
LT07-318 MycoAlert Detection Kit (100 Tests) Manual
LT07-418 MycoAlert Detection Kit (50 tests) Manual
LT07-701 MycoAlert PLUS detection kit (10 tests) Manual
LT07-703 MycoAlert PLUS detection kit (30 tests) Manual
LT07-705 MycoAlert PLUS detection kit (50 tests) Manual
LT07-710 MycoAlert PLUS detection kit (100 tests) Manual
LT07-518 MycoAlert Assay Control Set (10 tests) Manual
Interested in testing the MycoAlert Assay?

Contact us, and we will provide a free trail kit. 

More product information: 

• Brochure
• List of MycoAlert compatible luminometers (Please note ! If your plate reader is not on this list, you can still test its compatibility with a positive control dilution assay.)

Interested in knowing more?

Read about mycoplasma contamination in cell cultures, causes, problems and solutions. Click here.

Prices and ordering

Prices are available in our webshop. You may also contact us by e-mail for price information and ordering.

Lucetta™ 2 Luminometer

Interested in Using MycoAlert™ Assays but no luminometer at hand?

The Lucetta™ 2 luminometer is a single tube luminometer designed with a specific mode for using Lonza’s MycoAlert™ Mycoplasma Detection Assays. It guides you through the protocol and immediately provides assay results as CLEAN, BORDERLINE or CONTAMINATED sample.

The Lucetta™ 2 luminometer is provided by Lonza, and can also be used for many other luminescence-based assays, such as cell proliferation and cytotoxicity assays or luciferase reporter gene assays.

Lucetta™ 2 Luminometer
Kat. nr. AAL-1002  Lucetta™ 2 Luminometer Manual

Prevention and removal

Lonza offer several solution for mycoplasma elimination and prevention: 

MycoZap™ Mycoplasma Elimination Reagent

MycoZap™ Mycoplasma Elimination Reagent is an antibiotic formulation to remove Mycoplasma from contaminated cell cultures. The reagent clear detectable mycoplasma contamination in only 4 days with minimal toxic effects on the infected cells.

The MycoZap™ Reagent can be used to eradicate mollicutes, including Mycoplasma, Acholeplasma, Spiroplasma and Entomoplasma species in cell cultures.

Prevention and removal
LT07-818 MycoZap™ Mycoplasma Elimination Reagent (1 Treatment) Manual
LT07-918 MycoZap™ Mycoplasma Elimination Reagent (5 Treatments) Manual
MycoZap™ Plus-CL or Plus-PR

MycoZap™ Plus-CL and Plus-PR is a ready-to-use solution of antibiotics for protecting cell cultures from several types of common contaminants. It is active against mycoplasma, Gram-negative and Gram-positive bacteria, yeast and fungi. It can be used continuously and replaces Pen/strep.

MycoZap™ Plus-CL is used with continuous cell lines.

MycoZap™ Plus-PR is used with primary cells. It is reliable and gentle to primary cells, even though they are delicate and do not tolerate high antibiotic concentrations to prevent microbial growth.

VZA-2011 MycoZap™ Plus-CL (10 x 1 ml) Datasheet
VZA-2012 MycoZap™ Plus-CL (1 x 20 ml) Datasheet
VZA-2021 MycoZap™ Plus-PR (10 x 1 ml) Datasheet
VZA-2022 MycoZap™ Plus-PR (1 x 20 ml) Datasheet
MycoZap™ Prophylactic

MycoZap™ Prophylactic is a powerful combination of antibiotics that specifically prevents growth of mycoplasma in cell cultures. .

VZA-2031 MycoZap™ Prophylactic (10 x 1 ml) Datasheet
VZA-2032 MycoZap™ Prophylactic (1 x 20 ml) Datasheet
Prices and ordering

Prices are available in our webshop. You may also contact us by e-mail for price information and ordering. 

Contact person: Kristin Lundemo Sønsteby

Contact person: Kristin Lundemo Sønsteby