Mycoplasma detection

One of the most common contaminants in cell culture laboratories is mycoplasma. Cells are usually infected by cross-contamination from not tested infected cells (eg, through aerosols generated during pipetting, using the same medium bottles, working with more than one cell type at a time), contaminated material, contaminated donor tissue (<1%), or direct infection from the researcher.

Mycoplasma grows slowly and does not necessarily kill the cells, but affects different cellular parameters, such as changing the cell membrane's antigenicity, creating chromosomal level changes, inhibiting cell metabolism and fragmenting DNA.

Luminescens assay

The MycoAlert products from Lonza give you results in just 20 minutes!

The kits are a selective biochemical (luminescence) test that detects activity of any mycoplasmic enzymes. You perform a two-step reagent reaction with your cell supernatant, make two luminescence readings, and have your result in just 20 minutes.
The kits detect all the common mollicute contaminants (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma).

If you need a small luminometer we have Lucetta 2 Luminometer from Lonza. Small, light and very user-friendly, just insert the sample and do the reading.

And if you were to detect an infection, Lonza also offers MycoZap™ Reagent that can eliminate mycoplasma infections in just 4 days. This has been optimized to remove mycoplasma with minimal toxic effects on the infected cells.

Luminescens assay