Lonza
Mycoplasma detection
The MycoAlert™ Mycoplasma Detection Kit from Lonza, is a selective biochemical test that exploits the activity of mycoplasmal enzymes. These enzymes are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.
- Convenient and fast
- Simply add two reagents to the culture supernatant
- Perform two luminescence readings
- Results in less than 20 min
- Easy interpretation of results
- Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
- Negligible interference with media components
MycoAlert™ PLUS Mycoplasma Detection Kit generates a higher light output compared to the first-generation kit. This allows for:
- Use with less sensitive plate luminometers or multifunctional readers
- Testing of unused media, media supplements, or water
The positive control for the MycoAlert™ Mycoplasma Detection Kit is available separately in the MycoAlert™ Assay Control Set. The control set includes a lyophilized positive control and assay buffer for reconstitution, which also serves as a negative control. We strongly recommend using the control set when performing the mycoplasma detection assays.
| Cat. no. | Description | Size | Instructions |
|---|---|---|---|
| LT07-118 | MycoAlert Detection Kit | 10 tests | Download |
| LT07-218 | MycoAlert Detection Kit | 25 tests | Download |
| LT07-318 | MycoAlert Detection Kit | 100 tests | Download |
| LT07-418 | MycoAlert Detection Kit | 50 tests | Download |
| LT07-701 | MycoAlert PLUS detection kit | 10 tests | Download |
| LT07-703 | MycoAlert PLUS detection kit | 30 tests | Download |
| LT07-705 | MycoAlert PLUS detection kit | 50 tests | Download |
| LT07-710 | MycoAlert PLUS detection kit | 100 tests | Download |
| LT07-518 | MycoAlert Assay Control Set | 10 tests | Download |
Want to try it out on your cells?
Get one of the MycoAlert® Trial Kits, which include the MycoAlert® Positive Control Set.
TUTORIAL:
The MycoAlert® Mycoplasma Detection Kit tutorial – learn how to detect contamination in your cell culture
Mycoplasma contamination can be difficult to detect and can often go unnoticed for months. Mycoplasma can seriously impact the reliability, reproducibility, and consistency of experimental results. Simple routine testing for mycoplasma can help maintain the integrity of your cell culture research. This short tutorial demonstrates how the MycoAlert® Mycoplasma Detection Assay can help you detect mycoplasma contamination early.
Learn more about the MycoAlert® Mycoplasma Detection Kit and see how easy it is to use and integrate into your splitting routine.
BLOG ARTICLE: "What to do if you suspect Mycoplasma contamination in your cell culture"
Mycoplasma contamination can compromise the reliability of cell culture experiments and bioproduct manufacturing. Often undetected for months, it affects over 10% of cultures. Routine testing is essential to ensure uncontaminated work. Discover key challenges, detection methods, and prevention strategies in this blog post.
