Euroclone
N-GARDE mycoplasma PCR reagent set with internal control
Fast, sensitive and reliable mycoplasma detection for cell cultures
The N-GARDE Mycoplasma PCR Reagent Set is a highly sensitive and specific kit designed for the rapid detection of mycoplasma contamination in cell cultures. Mycoplasma contamination is a common and serious issue in biological research, affecting up to 30% of cell cultures and compromising the reliability and reproducibility of experimental results. Routine testing with N-GARDE ensures the integrity of your research and the safety of your biological products.
Key features:
- Rapid results: Complete detection protocol in approximately 5 hours.
- High sensitivity and specificity: Detects a broad range of mycoplasma species, including M. fermentans, M. hyorhinis, M. orale, M. salivarium, and more, as well as Acholeplasma and Spiroplasma species.
- Internal control included: Ensures test reliability by ruling out PCR inhibition and false negatives.
- Ready-to-use mix: All PCR reagents are pre-mixed and optimized, including loading dye for direct gel analysis.
- Easy protocol: Simple sample preparation and PCR setup—no need for radioisotope-labeled probes or complex reagent calculations.
- Research use only: Intended for research applications, not for diagnostic use.
How it works
The N-GARDE kit uses PCR amplification of a conserved, mycoplasma-specific 16S rRNA gene region. After amplification, results are visualized by agarose gel electrophoresis. Positive samples generate a 270 bp band, while the internal control appears at 357 bp, confirming the validity of each test.
Kit contents
- Reaction mix (200 μl)
- Internal control DNA template (20 μl)
- Internal control primers mix (100 μl)
- Buffer solution (1 ml)
- Positive template control (20 μl)
Storage & stability
Store at -20°C. The kit is stable for 36 months when kept in the dark under recommended conditions. Avoid repeated freeze-thaw cycles and prolonged exposure to light.
Applications
- Routine screening of cell cultures for mycoplasma contamination
- Quality control in research laboratories
- Ensuring the reliability of cell-based experiments
| Cat. no. | Description | Size | Package insert |
|---|---|---|---|
| EC-EMK090020 | N-GARDE Mycoplasma PCR Reagent set | 20 test | Download |
BLOG ARTICLE: "What to do if you suspect Mycoplasma contamination in your cell culture"
Mycoplasma contamination can compromise the reliability of cell culture experiments and bioproduct manufacturing. Often undetected for months, it affects over 10% of cultures. Routine testing is essential to ensure uncontaminated work. Discover key challenges, detection methods, and prevention strategies in this blog post.
