Agaroses
Agarose is a key component in many molecular biology workflows, providing a porous matrix for the separation, analysis and recovery of nucleic acids. By adjusting agarose type and concentration, you can optimize resolution, run time, and sample recovery for applications ranging from routine PCR checks to high‑resolution fragment analysis and pulsed‑field electrophoresis.
Different agarose formulations are tailored for:
- Routine DNA/RNA checks vs. high‑resolution analytical work
- Preparative gels for clean band excision vs. ultra‑sharp analytical separation
- Very small fragments vs. large genomic DNA and chromosomes
- Standard electrophoresis vs. PFGE and demanding downstream applications like blotting
Below is a product‑focused overview of our agaroses from Euroclone and Lonza, organized by application and key performance characteristics to help users quickly identify the most suitable agarose for their needs.
| Product | Main application / intended use | Fragment size range | Key properties & notes | Cat. no. | Size |
|---|---|---|---|---|---|
| PFGE & very large DNA | |||||
| GellyPhor® PFGE (Euroclone) | Analysis of high‑molecular‑weight nucleic acids (e.g. chromosomes) and large particles such as viruses and ribosomes | For high‑MW nucleic acids and large particles | Very high gel strength, low EEO, standard gelling temperature; designed for low‑percentage gels in PFGE. | EC‑EMR916100 | 100 g |
| SeaKem® Gold Agarose (Lonza) | PFGE, large fragment analysis | 1 kb – 10 Mb | High gel strength and performance‑certified; forms robust gels for large DNA fragments. | LZ-50152 | 25 g |
| LZ-50150 | 125 g | ||||
| LZ-50155 | 1 kg | ||||
| Routine analysis & blotting | |||||
| Agarose LE (Euroclone) | Nucleic acids routine screening electrophoresis and blotting | DNA separation 1–50 kb; DNA/RNA separation listed for standard gels | Multi‑purpose agarose with low EEO and standard melting point; optimized gel strength, sharp bands, high clarity and low background; RNase/DNase not detected. | EC‑EMR900500 | 500 g |
| SeaKem® LE Agarose (Lonza) | Routine DNA/RNA analysis, blotting | 100 bp – 23 kb | High gel strength agarose for robust, easy‑to‑handle gels in routine applications and blotting. | LZ-50001 | 25 g |
| LZ-50002 | 100 g | ||||
| LZ-50004 | 500 g | ||||
| LZ-50005 | 1 kg | ||||
| SeaKem® GTG® Agarose (Lonza) | DNA and RNA recovery, cloning | 100 bp – 23 kb | High gel strength and GTG® performance‑certified; combines robustness with suitability for recovery and downstream enzymatic work. | LZ-50071 | 25 g |
| LZ-50070 | 125 g | ||||
| LZ-50074 | 500 g | ||||
| LZ-50075 | 1 kg | ||||
| GellyPhor® LE (Euroclone) | General nucleic acids preparative and analytical electrophoresis; blotting; DNA fingerprinting; nucleic acid recovery | Supports DNA/RNA separation ≤1 kb and ≥1 kb | Low EEO for high electrophoretic mobility and shorter run times; high gel strength, low background; batch‑to‑batch consistent resolution; RNase/DNase not detected. | EC-EMR910100 | 100 g |
| EC-EMR910500 | 500 g | ||||
| EC-EMR910001 | 1 kg | ||||
| High Resolution – Small DNA Fragments (<1000 bp) | |||||
| NuSieve® 3:1 Agarose (Lonza) | PCR, blotting | 50 bp – 1 kb | High gel strength for small‑fragment PCR products and blots. | LZ-50091 | 25 g |
| LZ-50090 | 125 g | ||||
| LZ-50094 | 500 g | ||||
| LZ-50095 | 1 kg | ||||
| MetaPhor® Agarose (Lonza) | Fine resolution | 20 bp – 800 bp | Designed for fine resolution of small fragments; suited for applications needing precise sizing in the 20–800 bp range. | LZ-50181 | 25 g |
| LZ-50180 | 125 g | ||||
| LZ-50184 | 500 g | ||||
| LZ-50185 | 1 kg | ||||
| GellyPhor® HR (Euroclone) | Analytical electrophoresis with fine resolution of small DNA fragments | 10 bp – 1200 bp | Standard‑melting high‑resolution agarose; yields strong gels and finely resolves small DNA fragments; low EEO; high gel strength; RNase/DNase not detected. | EC-EMR912100 | 100 g |
| GellyPhor® ULTRA (Euroclone) | High‑resolution separation of PCR and RT‑PCR products; resolving closely sized fragments | 200 bp – 800 bp | Intermediate‑melting agarose with about twice the resolution of standard fine‑sieving agaroses; 2–4% gels approximate 4–8% polyacrylamide resolution; very low EEO and good gel strength. | EC-EMR915100 | 125 g |
| DNA/RNA Recovery (Low Melting Point – LMP) | |||||
| SeaPlaque® GTG® Agarose (Lonza) | In‑gel reactions, DNA and RNA recovery, cloning | 200 bp – 25 kb | Low melting temperature; GTG® performance‑certified for recovery, in‑gel reactions and downstream enzymatic applications. | LZ-50111 | 25 g |
| LZ-50110 | 125 g | ||||
| LZ-50115 | 1 kg | ||||
| SeaPlaque® Agarose (Lonza) | DNA and RNA recovery, cloning | 200 bp – 25 kb | Low melting temperature for convenient nucleic acid recovery and cloning from gels. | LZ-50101 | 25 g |
| LZ-50100 | 125 g | ||||
| LZ-50104 | 500 g | ||||
| LZ-50105 | 1 kg | ||||
| NuSieve® GTG® Agarose (Lonza) | DNA and RNA recovery, cloning | 50 bp – 1 kb | Low melting temperature; GTG® performance‑certified; optimized for small fragments where recovery and downstream reactions are critical. | LZ-50081 | 25 g |
| LZ-50080 | 125 g | ||||
| LZ-50084 | 500 g | ||||
| LZ-50085 | 1 kg | ||||
| GellyPhor® LM (Euroclone) | Preparative nucleic acids electrophoresis; cloning of tissue culture cells; viral plaque assays | DNA/RNA separation ≥1 kb |
Low melting temperature (≤65.5 °C at 1.5%) and low gelling temperature (24–28 °C at 1.5%); high clarity and greater sieving than standard agarose; ideal where band excision and downstream enzymatic reactions are needed. | EC-EMR911100 | 100 g |
| SeaPrep® Agarose | Cell cloning, cell embedding | Low‑melting agarose suitable for cell cloning and embedding applications. | LZ-50302 | 25 g | |
