Biotium
Nucleic acid gel stains
Biotium’s nucleic acid gel stains are designed as ultra-sensitive, safer replacements for ethidium bromide and SYBR stains in DNA and RNA gel electrophoresis. Choose ready-to-use pre-coated agarose kits, versatile GelRed®/GelGreen® formats, or visible stains like DNAzure® for UV‑free workflows.
GelRed® & GelGreen® classic gel stains
Drop‑in EtBr/SYBR alternatives for agarose and PAGE.
GelRed® is an ultra‑sensitive, extremely stable red fluorescent nucleic acid dye for dsDNA, ssDNA, and RNA in agarose or polyacrylamide gels, designed to replace highly toxic ethidium bromide. GelGreen® is a green fluorescent DNA gel stain more sensitive than SYBR Safe and compatible with blue‑light or UV imaging. Both can be used as post‑stains or precast in agarose and are available as concentrated stocks, ready‑to‑use solutions, loading dyes, and pre‑coated agarose.
EMBER™ Ultra DNA & RNA Gel Kits
Highest sensitivity with pre-coated agarose, no post-stain.
EMBER™ Ultra DNA and RNA Gel Kits use ultra‑pure agarose pre-coated with EMBER™ Ultra Dye plus dedicated loading dyes, giving superior sensitivity without a post‑electrophoresis staining step. DNA gels detect as little as ≤1 ng DNA, while RNA gels detect ≤5 ng RNA with excellent resolution. Kits are optimized for blue LED imagers (e.g. Gel‑Bright™) but are also compatible with UV transilluminators.
PAGE GelRed®
For DNA in polyacrylamide gels.
PAGE GelRed® is a non‑toxic, non‑mutagenic red DNA gel stain formulated for efficient penetration and staining of DNA in polyacrylamide gels, offering superior dsDNA sensitivity in PAGE and safe disposal compared to EtBr.
DNAzure® Visible Gel Stain
Blue bands by eye, no imager required.
DNAzure® Blue Nucleic Acid Gel Stain binds DNA in agarose gels and develops long‑lasting visible blue bands after 5–30 minutes of light exposure. It enables ultrasensitive detection (~1 ng DNA), simplifies band excision without UV damage, and bands remain stable for weeks and are detectable on near‑infrared scanners.
Classic Gel Stains portfolio
Biotium offers classic gel stains: Oxazole Gold (SYBR® Gold), a UV-excitable dye, is highly sensitive for DNA/RNA, especially RNA and single-stranded oligos. Thiazole Green (SYBR® Green) is UV/blue light-excitable and works for DNA/RNA in agarose or polyacrylamide gels. Ethidium bromide (EtBr), also UV-excitable, is widely used but is a known mutagen.
| Product | Application / suitable for | Cat. no. | Size |
|---|---|---|---|
| GelRed® 10,000X in water | EtBr‑replacement DNA/RNA stain; precast or post‑stain agarose; also post‑stain PAGE. | BIT-41003‑T | 0,1 ml |
| BIT-41003 | 0,5 ml | ||
| BIT-41003‑1 | 10 ml | ||
| GelGreen® 10,000X in water | Green DNA gel stain; more sensitive than SYBR Safe; blue‑light or UV imaging; precast or post‑stain. | BIT-41005 | 0,5 ml |
| BIT-41005‑1 | 10 ml | ||
| GelRed® 3X in water | Ready‑to‑use post‑electrophoresis staining solution for agarose (and post‑stain PAGE) gels. | BIT-41001 | 4 L |
| 6X GelRed® Prestain Loading Buffer, Orange Tracking Dye | Add directly to DNA samples for one‑step loading and prestaining on agarose gels. | BIT-41010 | 1 ml |
| GelRed® Prestain Plus 6X DNA Loading Dye | DNA prestain loading dye for fast visualization on GelRed®‑compatible agarose gels. | BIT-41011 | 1 ml |
| EMBER™ Ultra DNA Gel Kit | Highest‑sensitivity DNA staining in pre‑coated agarose; no post‑stain; blue LED or UV imaging. | BIT-41043-T | 10 gels |
| BIT-41043 | 30 gels | ||
| EMBER™ Ultra RNA Gel Kit | Highest‑sensitivity RNA staining in pre‑coated agarose; denaturing RNA loading dye; blue LED or UV. | BIT-41044-T | 10 gels |
| BIT-41044 | 30 gels | ||
| PAGE GelRed® 10,000X in water | Safer red DNA stain specifically formulated for polyacrylamide gels (PAGE). | BIT-41008‑T | 0,1 ml |
| BIT-41008‑500uL | 500 ul | ||
| PAGE GelRed® 1X in water | Ready‑to‑use 1X PAGE GelRed® post‑stain for nucleic acids in polyacrylamide gels. | BIT-41014 | 4 L |
| DNAzure® Blue Nucleic Acid Gel Stain, 100X | Visible blue DNA bands in agarose after light exposure; ultrasensitive (~1 ng DNA), UV‑free band excision. | BIT-41020 | 10 ml |
| Thiazole Green (SYBR® Green I), 10,000X in DMSO | BIT-40086-0.5mL | 500 uL | |
| BIT-40086-1mL | 1 mL | ||
| Oxazole Gold (SYBR® Gold), 10,000X in DMSO | BIT-40094 | 500 uL | |
| Ethidium Bromide, 10 mg/mL in H2O | BIT-40042 | 10 mL |
