IHC and IF workflow essentials
Reliable solutions for every step of your IHC and IF workflow - from slide preparation to detection and preservation. Explore our high-quality reagents and accessories designed to enhance staining accuracy and consistency
Coverslip sealant
CoverGrip™ Coverslip Sealant is the first product specifically designed for sealing the edges of wet-mounted coverslips for fluorescence microscopy. Unlike nail polish, CoverGrip™ contains no ingredients that can leach into aqueous mounting medium and affect specimen fluorescence.
Hydrophobic barrier PAP pen for tissue staining
ImmEdge® Hydrophobic barrier PAP Pen from Vector Laboratories is an improvement over the PAP pen for immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and in situ hybridization (ISH). It is designed to provide a heat-stable, water-repellent barrier that keeps reagents localized on tissue specimens and prevents mixing of reagents when multiple sections are mounted on the same slide.
Mounting media
Mounting media are essential for preserving tissue samples in immunohistochemistry (IHC) and immunofluorescence (IF). The right choice depends on factors like refractive index, viscosity, and compatibility with microscopy techniques. Vector Laboratories offers high-quality mounting media designed to enhance signal stability and sample longevity for reliable imaging.
CELLview™ products
Disposable plastic culture dishes, slides and plates with high-clarity glass for superior imaging. These products are ideal for high-resolution microscopy, providing consistent and reliable results for both routine and specialized cell culture applications.
BROCHURES & DOWNLOADS
Blocking Guide
Achieving clear immunohistochemistry (IHC) and immunofluorescence (IF) staining requires the right antibodies, detection system, and effective blocking. Proper blocking minimizes background, reduces non-specific binding, and improves target visualization. This guide provides practical solutions to troubleshoot and optimize your staining results.
Immunofluorescence Resource Guide
This guide offers you an overview of the IF workflow, optimization tips & tricks, and advice regarding autofluorescence (how to select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving).
Immunohistochemistry Resource Guide
This comprehensive guide starts with overview of the IHC workflow and proceeds to reference everything you need to know about IHC from choosing the right detection system to performing multiple antigen (multicolor/multiplex) labeling.
IHC Multiplexing Resource Guide
This resource is essential for those planning to localize two or more antigens within the same tissue section. It offers detailed protocols for chromogenic detection, supplemented by schematic illustrations of each procedure. Additionally, a comprehensive chart of all possible substrate pairings is included, along with photographic examples. Practical guidance on substrate sequencing, blocking strategies, and appropriate controls is also provided.
Staining Sample Preparation Guide
Achieving stunning immunohistochemistry (IHC) and immunofluorescence (IF) results requires careful planning and execution. This guide helps you optimize tissue morphology and minimize background by making informed choices at every step—from sample preparation to antigen retrieval.
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