Vector Laboratories

Human on Human & Mouse on Mouse

H.O.H.™ (Human on Human) Immunodetection Kit

The H.O.H. Immunodetection Kit is intended to detect human (or humanized) antibodies on frozen or paraffin embedded human tissue sections.

Key advantages of this Immunodetection Kit are:

  • Clear, crisp, specific staining of antigens of interest – no background
  • Procedures are simple and easy to follow
  • No tedious calculations are required

The H.O.H. Immunodetection Kit contains enough stock reagents to produce about 5 ml of working solution, which is generally sufficient to stain approximately 50 – 100 tissue sections depending on size.

White Paper: "Optimized Human on Human Immunodetection" 

H.O.H. (Human on Human) Immunodetection Kit

Cat. no.DescriptionSizeDatasheet
VEC-HOH-3000 H.O.H.™ (Human on Human) Immunodetection Kit1 kitDownload

Mouse On Mouse (M.O.M.) ImmPRESS Peroxidase Polymer Kit

Features:

  • Significant reduction of endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue
  • Clear, crisp specific staining of antigens of interest
  • Based on ImmPRESS reagent protocols
  • Procedures are simple and easy to follow
  • No tedious calculations or antibody prebinding steps required

M.O.M.® (Mouse on Mouse) ImmPRESS® Polymer Reagent

We also offer a M.O.M. ImmPRESS Peroxidase Polymer Reagent that is the same reagent as that contained in the Vector M.O.M. ImmPRESS Kit (MP-2400) and is meant for use with the Vector M.O.M. ImmPRESS Kit components. Reagents supplied in the Vector M.O.M Kits are matched reagents and titrated for the best possible performance.

For years, researchers attempting to localize mouse primary antibodies on mouse tissue have relied on the Vector M.O.M. Kit to produce crisp, strong, specific staining of antibody targets. The Vector M.O.M. Kits are specifically designed to significantly reduce endogenous mouse Ig staining when using mouse primary antibodies on mouse tissue. The inability of an Anti-Mouse detection antibody to distinguish between a primary antibody produced in mice and the endogenous mouse immunoglobulins present in mouse tissue results in high background staining which obscures specific staining. This problem can be essentially eliminated by using Vector M.O.M. Kits. The result is clear, crisp staining of the antigens of interest. Tedious calculations and primary antibody pre-binding steps are not required.

The Vector M.O.M. ImmPRESS Kit (MP-2400) contains our proprietary M.O.M. Mouse Ig Blocking Reagent paired with a specialized, ready-to-use, one-step M.O.M. ImmPRESS Peroxidase Polymer reagent (MPX-2402).  The M.O.M. ImmPRESS HRP polymer reagent is affinity purified and specifically optimized for use with the M.O.M. Mouse Ig Blocking Reagent.  The highly active peroxidase micro-polymers of the ImmPRESS Detection System provide outstanding sensitivity, limit steric interference, and enhance accessibility. Excellent staining results for a once difficult application have now become routine with the Vector M.O.M. System.

Cat. no.DescriptionSizeProduct information
VEC-MP-2400M.O.M.® (Mouse on Mouse) ImmPRESS®-HRP (Peroxidase) Polymer Kit1 kitDownload
VEC-MPX-2402-15M.O.M.® (Mouse on Mouse) ImmPRESS® Polymer Reagent, Anti-Mouse IgG, Peroxidase15 mlDownload

Reach out for more information!

 

Marte Sødal

Product manager

marte@bionordika.no

Marte Sødal

 

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