Vector Laboratories
VectaMount mounting media
Mounting media optimized for immunohistochemistry and immunocytochemistry
Using the correct mounting medium preserves your histochemical stains or precipitable enzyme substrates in tissue sections or cell preparations.
VectaMount® Express Mounting Medium
- A non-aqueous clearing and mounting medium designed for IHC-stained slides
- Eliminates the need for extensive ethanol washes and solvent-based clearing agents like xylene
- Fast-drying formula enables rapid visualization of stained samples
- Maintains stain integrity for at least 18 months at room temperature
- Compatible with horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme substrates, as well as common counterstains
- Optically clear with a refractive index of 1.49, ensuring high-quality imaging without distortion or artifacts
VectaMount AQ Aqueous Mounting Medium
- Hardsetting, aqueous mounting medium
- Preserves the color and clarity of enzyme substrates whose reaction products are soluble in alcohol or other organic solvents
- Ready-to-use formula - no mixing required
- Provides long-term stability, preserving stained sections for at least two years at room temperature when stored in a slide box
SUBSTRATE / VECTAMOUNT COMPATIBILITY TABLE
This table is designed as a reference to help you determine the optimal mounting medium for your application.
| Substrate | Cat. no. | VectaMount Express (#H-5700-60) | VectaMount AQ Aqueous (#H-5501-60) |
|---|---|---|---|
| HORSERADISH PEROXIDASE (HRP) | |||
| ImmPACT DAB (brown) | SK-4105 | ● | ● |
| ImmPACT DAB EqV (brown) | SK-4103-100 (100 ml) / SK-4103-400 (400 ml) | ● | ● |
| DAB (brown) | SK-4100 | ● | ● |
| DAB-Ni (gray-black) | SK-4100 | ● | |
| ImmPACT AEC (red) | SK-4205 | ● | |
| ImmPACT AMEC Red (red) | SK-4285 | ● | |
| AEC (red) | SK-4200 | ● | |
| ImmPACT VIP (purple) | SK-4605 | ● | |
| Vector VIP (purple) | SK-4600 | ● | |
| ImmPACT SG (blue-gray) | SK-4705 | ● | ● |
| Vector SG (blue-gray) | SK-4700 | ● | ● |
| ImmPACT NovaRED (red) | SK-4805 | ● | |
| Vector NovaRED (red) | SK-4800 | ● | |
| ALKALINE PHOSPHATASE (AP) | |||
| ImmPACT Vector Red (magenta) | SK-5105 | ● | ● |
| Vector Red (magenta) | SK-5100 | ● | ● |
| Vector Blue (blue) | SK-5300 | ● | ● |
| BCIP / NBT (indigo) | SK-5400 | ● | ● |
This comprehensive guide starts with overview of the IHC workflow and proceeds to reference everything you need to know about IHC from choosing the right detection system to performing multiple antigen (multicolor/multiplex) labeling.
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