Plasmid DNA purification
Monarch Spin Plasmid Miniprep Kit
- Achieve highly concentrated DNA with elution volumes as low as 30 μl and yields up to 20 μg.
- Optimized column design prevents buffer retention and salt carryover, ensuring high purity.
- Color-coded buffer system simplifies the workflow by visually indicating key process completion.
- Faster protocols reduce hands-on time and minimize spin steps for improved efficiency.
- Eco-friendly design with significantly reduced plastic usage in columns and kit compared to top competitors.
The Monarch Spin Plasmid Miniprep Kit provides an advanced solution for plasmid DNA extraction and purification from bacterial cultures, delivering up to 20 μg of high-quality DNA. The upgraded spin columns are engineered for low elution volumes, enabling highly concentrated DNA recovery in as little as 30 μl, making it ideal for applications such as sequencing, restriction digestion, transformation, and transfection. A color-coded buffer system ensures clear visualization of lysis and neutralization steps, streamlining the workflow and reducing processing time. The kit is designed for sustainable lab practices, utilizing minimal plastic while maintaining structural integrity for centrifugation and vacuum manifolds. Monarch spin columns are manufactured using state-of-the-art automation to ensure high precision and reliability for every run.
Properties:
| Purification format: | Spin column for nucleic acid purification |
| Compatible methods: | Centrifugation or vacuum manifold |
| Intended usage: | Plasmid extraction and preparation from various E.coli host strains and other microbial strains |
| Plasmid DNA yield: | Up to 20 μg* |
| Culture volume: | 1-5 ml, not to exceed 15 OD units |
| Column binding capacity: | Up to 20 μg |
| Plasmid size: | Up to 25 kb |
| Elution volume: | ≥ 30 μl |
| DNA purity: | A260/280 > 1.8 and A260/230 > 1.8 |
| Compatible: downstream: applications | Restriction digestion and other enzymatic manipulations, transformation, transfection, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc. |
* Yield depends on plasmid copy number, host strain, culture volume and growth conditions
Product listing:
| Cat. no. | Description | Size | Manual |
|---|---|---|---|
| NEB-T1110S | Monarch® Spin Plasmid Miniprep Kit | 50 preps | Download |
| NEB-T1110L | Monarch® Spin Plasmid Miniprep Kit | 250 preps | Download |
