RNA isolation
Monarch® Spin RNA Isolation Kit (Mini)
- Purify up to 100 µg of high-quality total RNA from diverse sample types, including difficult-to-lyse samples, with this all-in-one kit
- Extract RNA from cells, tissues (fibrous or lipid-rich), Gram-positive and Gram-negative bacteria, yeast, plants, insects, and more; includes Proteinase K for sample processing and Monarch StabiLyse™ DNA/RNA Buffer for sample preservation
- Combines the high binding capacity of a standard mini kit (100 µg) with the low elution volume of a micro kit (10 µl)
- Effectively remove genomic DNA contamination using specialized columns and RNase-free DNase, included in the kit
- Capture the total RNA pool, including small RNAs <200 nt, for comprehensive RNA analysis
The Monarch Spin RNA Isolation Kit (Mini) is a complete solution for sample preservation, cell lysis, gDNA removal, and total RNA purification from a variety of biological samples, including cultured cells, mammalian tissues, microbes, plants, insects, and blood.
With just one kit, users can purify up to 100 µg of high-quality total RNA from both standard and tough-to-lyse samples, using the included enzymes, reagents, and specialized gDNA removal columns. This kit uniquely combines the high binding capacity of RNA miniprep kits with the low elution volumes of micro kits, enabling efficient RNA recovery. It also supports cleanup of enzymatic reactions and purification from TRIzol®-extracted samples.
Purified RNA has A260/280 and A260/230 ratios typically >2.0, high RNA integrity scores, and minimal residual gDNA. The kit captures RNA ranging from full-length rRNAs to intact miRNAs, making it ideal for applications such as RT-qPCR, cDNA synthesis, RNA-seq, and RNA hybridization-based technologies.
Designed for sustainability, Monarch kits use significantly less plastic than other market alternatives.
Properties:
| Purification format: | Spin column for nucleic acid purification |
| Compatible methods: | Centrifugation or vacuum manifold |
| Intended usage: | Total RNA isolation |
| Compatible sample types: | Cells, microbes, plants, insects, mammalian tissues including soft, fibrous and lipid-rich, blood, plasma |
| Yield: | Varies depending on sample type |
| Column binding capacity: | Up to 100 μg total RNA |
| RNA size: | ≥ 20 nt, total RNA including small RNA, miRNA, mRNA, lncRNA, rRNA |
| Elution volume: | ≥ 10 μl |
| RNA purity: | Typically A260/280 ≥ 2.0 and A260/230 ≥ 1.8 |
| Compatible downstream applications: | cDNA synthesis, RT-qPCR, RNA-Seq, small RNA library prep, Northern blotting, hybridization-based workflows |
Product listing:
| Cat. no. | Description | Size | Manual |
|---|---|---|---|
| NEB-T2110S | Monarch® Spin RNA Isolation Kit (Mini) | 50 preps | Download |
