Q5^® high-fidelity DNA polymerase products

NEBNext^® Ultra™ II Q5^® Master Mix (#M0544)

NEBNext Ultra II Q5^® Master Mix is the most recent NEBNext formulation of Q5 High Fidelity DNA Polymerase, optimized for amplification of NGS libraries. This hot start formulation is designed for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, and further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.

NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.

• Optimized for high yields in NGS library amplification
• Minimizes GC bias, with superior performance across the GC spectrum
• Ultra-high-fidelity amplification with Q5, the highest-fidelity polymerase (1)
• Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
• Bulk format available: M0544X = 500 reactions in 1 x 12.5 ml bottle
   

Download the NEBNext technical guide for Illumina 

50 rxns (50 µl vol)
250 rxns (50 µl vol)
500 rxns (50 µl vol)

NEBNext^® Q5U^® Master Mix (#M0597)

NEBNext^® Q5U^® Master Mix contains a modified version of Q5^® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. 
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.

• Optimized for high yields in uracil-containing NGS library amplification
• Compatible with NEBNext Enzymatic Methyl-seq (EM-seq™) and bisulfite sequencing workflows
• Minimizes GC bias, with superior performance across the GC spectrum
• Ultra-high-fidelity amplification
• Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
   

Download the NEBNext technical guide for Illumina 

50 rxns
250 rxns

Q5^® High-Fidelity 2X Master Mix (#M0492)

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

• Highest fidelity amplification (~280X higher than Taq)
• Master mix includes Q5 High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Also available: Q5 Hot Start standalone polymerase (#M0493) and hot start master mix formats (#M0494)
   

100 rxns
500 rxns

Q5^® High-Fidelity DNA Polymerase (#M0491)

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. 

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Also available: Q5 Hot Start standalone polymerase (#M0493) and master mix formats (#M0492, M0494)
   

100 units
500 units

Q5^® High-Fidelity PCR Kit (#E0555)

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Hot Start and Master Mix formats available
   

50 rxns
200 rxns

Q5^® Hot Start High-Fidelity 2X Master Mix (#M0494)

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

• Highest fidelity amplification (~280X higher than Taq)
• Master mix includes Q5 Hot Start High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Bulk format available: M0494X = 1 x 12.5 ml bottle
   

100 rxns (2x1.25ml)
500 rxns (10x1.25ml)
500 rxns (1x12.5ml)

Q5^® Hot Start High-Fidelity DNA Polymerase (#M0493)

Q5^® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Also available: Q5 standalone polymerase (#M0491) and master mix formats (#M0492, #M0494)
   

100 units
500 units

Q5U^® Hot Start High-Fidelity DNA Polymerase (#M0515)

Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5^® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

• Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
• Prevent carryover contamination when combined with dUTP (#N0459) and Uracil DNA Glycosylase (UDG) (#M0372) treatment
• Generate higher assembly efficiency and improve accuracy in USER^® cloning
• Enable room temperature setup with aptamer-based hot start formulation
• Utilize optimized protocols for recommended applications
• Reads and incorporates uracil and inosine bases
   

100 units
500 units

Q5^® Blood Direct 2X Master Mix (#M0500)

• Skip DNA purification and amplify direct from human whole blood cells or dried blood spots
• Utilize Q5 Hot Start High-Fidelity DNA Polymerase for accurate and efficient PCR
• Benefit from increased resistance to inhibitors and chemicals with our optimized buffer system
• Set up reactions at room temperature with aptamer-based enzyme control
   

100 rxns
500 rxns