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NEW ENGLAND BIOLABS

Q5® high-fidelity DNA polymerase products

Q5® high-fidelity DNA polymerase sets a new standard for both fidelity and robust performance. 

FEATURES:

  • Extremely low error rates
    At ~280X higher than Taq, Q5 offers unparalleled fidelity for your most important samples.
  • Robust amplification with minimal optimization
    High specificity and yield are absolute requirements for today's molecular biology techniques.
  • Superior coverage for a broad range of amplicons, regardless of GC content
    While other DNA polymerases can have difficulty amplifying high-GC or high-AT amplicons, Q5 displays superior performance for a wide range of templates.
  • Shorter PCR protocols
    Achieve precision without sacrificing speed. Q5's unique design incorporating the SSo7d processivity-enhancing domain enables shorter extension times, as low as 10 seconds per kb. Additionally, aptamer-based hot start requires no initial denaturation step and enables room temperature setup.
  • Amplify templates up to 20 kb
    With Q5, you can reliably amplify simple templates up to 20 kb. Complex templates up to 10 kb can also be amplified with a high degree of confidence.

REFERENCE

“We are using NEBNext® Q5U® Master Mix to amplify sequencing library pools after hybridization capture and bisulfite conversion. It's a tough job for an enzyme, but Q5U® does it well, and the quality of sequencing data is excellent."

Oleksii Nikolaienko
Researcher, Mohn Kreftforskningslaboratorium

Cat. no.ProductSize
NEB-M0578LQ5 Quick-Load High-Fidelity 2X Master Mix500 rxns
NEB-M0580LQ5 Quick-Load Hot Start High-Fidelity 2X Master Mix500 rxns
NEB-M0544SNEBNext Ultra II Q5 Master Mix 50 rxns (50 µl vol)
NEB-M0544LNEBNext Ultra II Q5 Master Mix250 rxns (50 µl vol)
NEB-M0597SNEBNext Q5U Master Mix50 rxns (50 µl vol)
NEB-M0597LNEBNext Q5U Master Mix250 rxns (50 µl vol)
NEB-M0492SQ5 High-Fidelity 2X Master Mix100 rxns (50 µl vol)
NEB-M0492LQ5 High-Fidelity 2X Master Mix 500 rxns (50 µl vol)
NEB-M0491SQ5 High-Fidelity DNA Polymerase100 units
NEB-M0491LQ5 High-Fidelity DNA Polymerase500 units
NEB-E0555SQ5 High-Fidelity PCR Kit50 rxns
NEB-E0555LQ5 High-Fidelity PCR Kit200 rxns
NEB-M0494SQ5 Hot Start High-Fidelity 2X Master Mix100 rxns (2x1.25ml) 
NEB-M0494LQ5 Hot Start High-Fidelity 2X Master Mix500 rxns (10x1.25ml)
NEB-M0493S Q5 Hot Start High-Fidelity DNA Polymerase100 units
NEB-M0493LQ5 Hot Start High-Fidelity DNA Polymerase500 units
NEB-M0515SQ5U Hot Start High-Fidelity DNA Polymerase100 units              
NEB-M0515L Q5U Hot Start High-Fidelity DNA Polymerase500 units 
NEB-M0500S Q5 Blood Direct 2X Master Mix 100 rxns (1x1ml)              
NEB-M0500L Q5 Blood Direct 2X Master Mix 500 rxns (5x1ml)              

NEBNext® Ultra™ II Q5® Master Mix (#M0544)

NEBNext Ultra II Q5® Master Mix is the most recent NEBNext formulation of Q5 High Fidelity DNA Polymerase, optimized for amplification of NGS libraries. This hot start formulation is designed for robust, high-fidelity amplification of next-generation sequencing (NGS) libraries, and further improves the uniformity of amplification of libraries, including superior performance with GC-rich regions.

NEBNext Ultra II Q5 Master Mix is also included as a component in all NEBNext Ultra II kits, for DNA and RNA library preparation.

  • Optimized for high yields in NGS library amplification
  • Minimizes GC bias, with superior performance across the GC spectrum
  • Ultra-high-fidelity amplification with Q5, the highest-fidelity polymerase (1)
  • Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
  • Bulk format available: M0544X = 500 reactions in 1 x 12.5 ml bottle
     

Download the NEBNext technical guide for Illumina 

50 rxns (50 µl vol)
250 rxns (50 µl vol)
500 rxns (50 µl vol)

NEBNext® Q5U® Master Mix (#M0597)

NEBNext® Q5U® Master Mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification. 
NEBNext Q5U Master Mix is included as a component in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) and is also compatible with library amplification in bisulfite sequencing workflows.

  • Optimized for high yields in uracil-containing NGS library amplification
  • Compatible with NEBNext Enzymatic Methyl-seq (EM-seq™) and bisulfite sequencing workflows
  • Minimizes GC bias, with superior performance across the GC spectrum
  • Ultra-high-fidelity amplification
  • Aptamer-based hot start for room-temperature reaction set-up without a separate activation step
     

Download the NEBNext technical guide for Illumina 

50 rxns
250 rxns

Q5® High-Fidelity 2X Master Mix (#M0492)

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Master mix includes Q5 High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Also available: Q5 Hot Start standalone polymerase (#M0493) and hot start master mix formats (#M0494)
     

100 rxns
500 rxns

Q5® High-Fidelity DNA Polymerase (#M0491)

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. 

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Also available: Q5 Hot Start standalone polymerase (#M0493) and master mix formats (#M0492, M0494)
     

100 units
500 units

Q5® High-Fidelity PCR Kit (#E0555)

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot Start and Master Mix formats available
     

50 rxns
200 rxns

Q5® Hot Start High-Fidelity 2X Master Mix (#M0494)

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Master mix includes Q5 Hot Start High-Fidelity DNA Polymerase, dNTPs, and Mg++ in a broad-use buffer
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Bulk format available: M0494X = 1 x 12.5 ml bottle
     

100 rxns (2x1.25ml)
500 rxns (10x1.25ml)
500 rxns (1x12.5ml)

Q5® Hot Start High-Fidelity DNA Polymerase (#M0493)

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (#M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Also available: Q5 standalone polymerase (#M0491) and master mix formats (#M0492, #M0494)
     

100 units
500 units

Q5U® Hot Start High-Fidelity DNA Polymerase (#M0515)

Q5U Hot Start High-Fidelity DNA Polymerase is a modified version of Q5® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

  • Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
  • Prevent carryover contamination when combined with dUTP (#N0459) and Uracil DNA Glycosylase (UDG) (#M0372) treatment
  • Generate higher assembly efficiency and improve accuracy in USER® cloning
  • Enable room temperature setup with aptamer-based hot start formulation
  • Utilize optimized protocols for recommended applications
  • Reads and incorporates uracil and inosine bases
     

100 units
500 units

Q5® Blood Direct 2X Master Mix (#M0500)

  • Skip DNA purification and amplify direct from human whole blood cells or dried blood spots
  • Utilize Q5 Hot Start High-Fidelity DNA Polymerase for accurate and efficient PCR
  • Benefit from increased resistance to inhibitors and chemicals with our optimized buffer system
  • Set up reactions at room temperature with aptamer-based enzyme control
     

100 rxns
500 rxns