NGS library quantitation

The BrightBox Assay is a novel method designed to quantitate NGS libraries efficiently. Traditional library quantitation can be a lengthy, costly, and inaccurate process, often resulting in variability that leads to unequal library loading, wasting both time and money.

The BrightBox Assay offers a quick and simple solution to accurately quantitate any NGS library. Additionally, it allows you to get results quickly by shortening your current library preparation and instrument loading workflows.
 

Why use BrightBox:

• FAST
  • Save time with reactions completed in just 5 minutes
  • Assemble reactions quickly with reagents stored at 4 degrees
     
• EASY
  • Quantitate any NGS library made for Illumina sequencers
  • Only detects sequenceable fragments with P5 and P7 adapters
  • Works on any qPCR instrument or plate reader
  • Reduce manual errors and save time with a premade Assay Mix and prediluted standards kept in refrigerated storage
     
• ACCURATE
  • Reduce manual errors and save time with a premade single Assay Mix
  • Use the five prediluted standards and negative control included in the kit
  • Improve data with consistent pooling based on actual molarity
     
• FLEXIBLE
  • Improve data with consistent pooling based on actual molarity and a single method for quantitation
  • Linear across a large dynamic range
  • Quantitate any library with attached P5 and P7 adapters​