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Articles
Advancing 3D cell culture: How Gelomics platforms help you model human biology more realistically
2D cell culture is a limited model for mimicking real biology. Usually, only one cell type is cultured. An alternative with ethical issues, and in many cases, not reflecting human physiology would be using animal models. Both FDA and EMA are working on phasing out animal experiments and the future is doing tests in 3D cell cultures, organoids and tissue culture.
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Considerations related to purity and quality control of isolated nucleic acids
Learn about key considerations for assessing nucleic acid purity and quality. Discover why absorbance ratios (A260/280, A260/230) have limitations and why complementary methods and functional validation are essential for accurate evaluation.
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Contaminants in your nucleic acid sample?
Contaminants in nucleic acid samples can compromise your research results, leading to inaccurate data and wasted resources. In this article, we delve into the common sources of contamination, their impact on your experiments, and effective strategies to ensure the purity of your samples.
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Basics of Bioconjugation
Bioconjugation is the establishment of covalent bonds between a biomolecule, eg. a protein, a carbohydrate, a lipid, or a nucleic acid, and another molecule or material. This method is used in manufacturing of ELISA, ELISpot, WB, IHC, IF, or other antibody-based methods, such as Antibody-drug conjugates (ADCs). Bioconjugation modifies the structures of the biomolecules, giving them new functions that they cannot exhibit by themselves.
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Nurturing complexity: The role of vascularization in organoid advancements
Explore the essential role of vascularization in organoid advancements. Learn about cutting-edge methods like co-culturing, bioprinting, and organoids-on-a-chip. Discover how these techniques are reshaping medical research. Read more now!
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Ta vare på din PIPETBOY
Siden de ble tilgjengelige for over førti år siden har Pipetboy vært de mest populære pipettefyllerne på markedet. De finnes på mange laboratorier, ofte i ulike modeller og generasjoner, gamle som nye. Med mye bruk kommer slitasje, så kanskje behøver deres Pipetboy en kjapp vedlikeholdsrunde?
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"In house"-veileder 2023.
Den etterlengtede «MDCG 2023-1 Guidance on the health institution exemption under Article 5(5) of Regulation (EU) 2017/745 and Regulation (EU) 2017/746» ble publisert i begynnelsen av januar 2023. I denne blogposten Kommenterer kvalitetskoordinator Espen Kibsgård og Rolf A. Klaasen ved Klinikk for laboratoriemedisin, Oslo universitetssykehus sentrale kapitler i veilederen. Disse kommentarene har også blitt diskutert med medlemmer i IVDR prosjektgruppen i Helse Sør-Øst.
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What to do if you suspect Mycoplasma contamination in your cell culture
Mycoplasma contamination poses a common and often undetected threat in cell cultures, affecting around 10% of them. This blog article delves into the challenges of detecting, eliminating, and preventing mycoplasma contamination. It offers insights into best practices and answers common questions to assist researchers in addressing this widespread issue.
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Having trouble when amplifying GC-rich sequences? Here are four tips on how to troubleshoot
GC-rich templates in DNA sequences have 60% or more G (guanine) or C (cytosine) bases. Amplifying GC-rich templates may require adjusting reagents, annealing temperature, and using specialized master mixes or standalone polymerases for optimal results, and in this blog article you get four tips on how to troubleshot.
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How to evaluate your qPCR and RT-qPCR results
qPCR and RT-qPCR methods are described, their usefulness and power as well as their sensitivity and vulnerability. In addition, it is explained how qPCR evaluation is accomplished by applying the “MIQE” guidelines and NEB´s “dots in boxes” quality scoring methods.
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