Lonza
Serum-free X‑VIVO® and TheraPEAK™ X‑VIVO™ media for immune cell culture
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X‑VIVO® cell culture media from Lonza (X‑VIVO® 10, 15 and 20) are serum‑free media developed for culturing different types of immune cells. They are widely used in immunology research and cell therapy workflows, including T‑cell, TIL, NK and LAK cell cultures, as well as CAR‑T development and optimization.
For applications that require GMP‑compatible raw materials, TheraPEAK™ X‑VIVO™ media provide GMP‑grade versions of X‑VIVO 10 and X‑VIVO 15. All TheraPEAK products are produced according to applicable GMP standards and follow USP/EP guidance for cell and gene therapy raw materials. They are intended to support GMP manufacturing and are produced at FDA‑registered sites operating under an ISO 13485‑certified quality management system.
The overviews below can help you choose the right medium and format for your application.
Request your tailored X-VIVO® offer.
Attractive volume-based pricing available!
The offer is valid until 31.03.2026.
Media selection guide (by medium)
| Medium | Main applications | Typical cell types | Key points |
|---|---|---|---|
| X‑VIVO® 10 (RUO) | LAK generation and serum‑free culture of hematopoietic cells in adoptive immunotherapy and cell therapy research. | LAK cells, peripheral blood lymphocytes (PBL), hematopoietic stem cells, monocytes, macrophages. | Serum‑free LAK medium containing human albumin, insulin and transferrin. Often used for original LAK protocols in a serum‑free environment. |
| X‑VIVO® 15 (RUO) | Immunology and cell therapy R&D; optimized for TIL and CD3+ T‑cell proliferation; widely used in CAR‑T workflows. | CD3+ T cells, NK cells, PBL, tumor infiltrating lymphocytes (TILs), monocytes, macrophages, granulocytes, dendritic cells, HUT‑78 and related cell lines. | Versatile “workhorse” medium for many immune cell types. Available in several formulations (with/without recombinant transferrin, different protein levels). |
| X‑VIVO® 20 (RUO) | High‑density culture of LAK cells from monocyte‑depleted PBL; also used for PBL and TIL cultures. | High‑density LAK cells, PBL, tumor infiltrating lymphocytes (TILs). | Chosen when cell density and performance in LAK cultures are critical. |
| TheraPEAK™ X‑VIVO™ 10 (GMP‑grade) | GMP‑compatible medium for hematopoietic cells and LAK generation, typically used as raw material in cell and gene therapy manufacturing. | Dendritic cells, lymphocytes, monocytes, macrophages, granulocytes, hematopoietic stem cells, NK cells, LAK cells. | TheraPEAK (GMP‑grade) version of X‑VIVO 10. Antibiotic‑free, phenol‑red‑free, xenofree formulations with native or recombinant transferrin. |
| TheraPEAK™ X‑VIVO™ 15 (GMP‑grade) | GMP‑grade T‑cell and TIL medium for cell and gene therapy workflows, including CAR‑T manufacturing. | Purified CD3+ cells from peripheral blood or tumors, NK cells, PBL, monocytes, macrophages and other hematopoietic cells. | TheraPEAK (GMP‑grade) X‑VIVO 15 with options for native or recombinant transferrin, bottles or bags, all without phenol red or gentamicin. |
All formats and catalog numbers
Use this overview to find the right catalog number and formulation (RUO vs TheraPEAK/GMP‑grade, with or without gentamicin and phenol red, native vs recombinant transferrin, bottle vs bag).
| Cat. no | Medium | Size | Main applications | Typical cell types | Key points |
|---|---|---|---|---|---|
| X‑VIVO® media (RUO) | |||||
| 04‑380Q | X‑VIVO® 10, serum‑free hematopoietic cell medium | 1 L | LAK generation and serum‑free culture of hematopoietic cells in adoptive immunotherapy and cell therapy research. | LAK cells, PBL, hematopoietic stem cells, monocytes, macrophages. | Contains L‑glutamine, HEPES, recombinant human insulin, gentamicin and phenol red, plus pharmaceutical‑grade human albumin and transferrin. Original protein‑containing X‑VIVO 10, not antibiotic‑free or phenol‑red‑free. |
| 02‑060F | X‑VIVO® 15, serum‑free hematopoietic cell medium | 500 ml | Immunology and cell therapy R&D; optimized for TIL and CD3+ T‑cell proliferation under serum‑free conditions | CD3+ T cells, NK cells, PBL, monocytes, macrophages, granulocytes, HUT‑78 and related human lymphocytic cell lines. | With L‑glutamine, gentamicin and phenol red (xenofree). Standard X‑VIVO 15 formulation, contains antibiotics and indicator dye. |
| 02‑060Q | X‑VIVO® 15, serum‑free hematopoietic cell medium | 1 L | Immunology and cell therapy R&D; optimized for TIL and CD3+ T‑cell proliferation under serum‑free conditions | CD3+ T cells, NK cells, PBL, monocytes, macrophages, granulocytes, HUT‑78 and related human lymphocytic cell lines. | With L‑glutamine, gentamicin and phenol red (xenofree). Standard X‑VIVO 15 formulation, contains antibiotics and indicator dye. |
| 02‑053Q | X‑VIVO® 15, serum‑free hematopoietic cell medium with recombinant transferrin | 1 L | Same TIL and T‑cell applications as other X‑VIVO 15 formulations, with recombinant transferrin for xeno‑free workflows. | CD3+ T cells, NK cells, PBL, monocytes, macrophages and related hematopoietic cells. | With L‑glutamine, gentamicin, recombinant transferrin and phenol red. Good when recombinant (xeno‑free) transferrin is desired but antibiotics/indicator are acceptable. |
| 04‑418Q | X‑VIVO® 15, chemically defined serum‑free hematopoietic cell medium | 1 L | Serum‑free expansion of TIL and other lymphocytes; suitable for investigation of second messenger systems (no PKC stimulators). | NK cells, TIL, PBL, stem cells, monocytes, macrophages, HUT‑78. | Complete chemically defined formulation containing pharmaceutical‑grade human albumin, recombinant human insulin and pasteurized human transferrin, with gentamicin and phenol red. Higher protein content than 02‑060, not antibiotic‑free. |
| 04‑448Q | X‑VIVO® 20, serum‑free hematopoietic cell medium | 1 L | Optimized for generation of LAK cells from monocyte‑depleted PBL at high density; also used as growth medium for PBL and TIL. | High‑density LAK cells, PBL, tumor infiltrating lymphocytes (TILs). | X‑VIVO 20 with L‑glutamine, gentamicin and phenol red. Chosen when very high‑density LAK cultures are needed and antibiotics/indicator are acceptable |
| TheraPEAK™ X-VIVO™ media (GMP‑grade) | |||||
| BP04‑743Q | TheraPEAK™ X‑VIVO™ 10, serum‑free hematopoietic cell medium | 1 L | GMP‑compatible medium for hematopoietic cells and LAK generation, where antibiotics and phenol red are avoided. | Dendritic cells, lymphocytes, monocytes, macrophages, granulocytes, LAK, PBL, stem cells. | With L‑glutamine, without gentamicin and phenol red, xenofree. Includes human transferrin. Good step when moving X‑VIVO 10 processes towards GMP |
| BEBP04‑743Q | TheraPEAK™ X‑VIVO™ 10, serum‑free hematopoietic cell medium | 1 L | Same applications as BP04‑743Q in fully GMP‑labelled BE‑code. | Hematopoietic cells including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes. | With L‑glutamine, without gentamicin and phenol red, xenofree. Preferred when customers explicitly request TheraPEAK / BE‑code GMP products. |
| BEBP02‑055Q | TheraPEAK™ X‑VIVO™ 10 with recombinant transferrin | 1 L | GMP manufacturing of slower‑growing, less mature hematopoietic cells, where recombinant (xeno‑free) transferrin is preferred. | Hematopoietic stem cells, NK cells, LAK cells and other hematopoietic cells. | With L‑glutamine, without gentamicin or phenol red, contains recombinant transferrin. Best choice when both GMP‑grade and recombinant transferrin are required. |
| BEBP02‑061Q | TheraPEAK™ X‑VIVO™ 15, serum‑free hematopoietic cell medium | 1 L | GMP‑compatible T‑cell and TIL processes where antibiotics and phenol red are not desired. | Variety of hematopoietic cells (e.g. T cells, NK cells, PBL, monocytes, macrophages). | With L‑glutamine, without gentamicin and phenol red, xenofree. Good “default” TheraPEAK X‑VIVO 15 GMP medium. |
| BP02‑054Q | TheraPEAK™ X‑VIVO™ 15 with recombinant transferrin | 1 L | GMP manufacturing of T cells/TILs where recombinant transferrin is preferred and antibiotics/indicator are avoided. | Same hematopoietic cell spectrum as other X‑VIVO 15 (CD3+ T cells, NK, PBL, monocytes, etc.). | With L‑glutamine, without gentamicin and phenol red, contains recombinant transferrin. Highlight as GMP + recombinant transferrin option. |
| BE08‑879H | TheraPEAK™ X‑VIVO™ 15 with native human transferrin (GMP‑grade) | 5 L bag | Larger‑scale GMP manufacturing in bag format; transferrin‑supplemented medium without gentamicin or phenol red. | Same TheraPEAK X‑VIVO 15 cell types as above (T cells, NK cells, PBL, monocytes, etc.). | With native human transferrin, no phenol red, no gentamicin, 5 L bag. Ideal for scale‑up and closed systems. |
| BE08‑879P1 | TheraPEAK™ X‑VIVO™ 15, serum‑free hematopoietic cell medium (GMP‑grade) | 1 L bag | GMP workflows using bag‑based / closed systems where an antibiotic‑free, phenol‑red‑free T‑cell medium is needed. | Same hematopoietic cell spectrum as other TheraPEAK X‑VIVO 15 media. | With L‑glutamine, without gentamicin and phenol red, xenofree, 1 L bag. Good when moving from bottles to bags at smaller scale. |
Media selection support and quote request
Do you need support in media selection or would you like to get a quote of X‑VIVO® media?
Contact our product manager Målfrid (malfrid@bionordika.no) for advice and pricing, and we will help you select the most suitable medium for your process.
Reach out for more information!
