New England Biolabs
A supplier-of-choice for scientists across the globe, NEB offers the largest selection of recombinant and native enzymes for genomic research. While restriction enzymes remain part of our core product portfolio, our ever-expanding catalog also includes products related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis.
Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors, including molecular diagnostics development.
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Knowledge and insights from the Science Hub
Articles
Having trouble when amplifying GC-rich sequences? Here are four tips on how to troubleshoot
GC-rich templates in DNA sequences have 60% or more G (guanine) or C (cytosine) bases. Amplifying GC-rich templates may require adjusting reagents, annealing temperature, and using specialized master mixes or standalone polymerases for optimal results, and in this blog article you get four tips on how to troubleshot.
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How to evaluate your qPCR and RT-qPCR results
qPCR and RT-qPCR methods are described, their usefulness and power as well as their sensitivity and vulnerability. In addition, it is explained how qPCR evaluation is accomplished by applying the “MIQE” guidelines and NEB´s “dots in boxes” quality scoring methods.
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