NEBNext UltraExpress^TM Library Prep Kit for DNA and RNA

The NEBNext UltraExpress Library Prep Kit is the latest generation of NEBNext DNA and RNA library prep, with a fast, streamlined workflow that enables the creation of high-yield, high-quality libraries. 

How fast? 
DNA: < 2 hours 
RNA: 3 hours 

Besides speed, the NEBNext UltraExpress Library Prep Kits also provide other advantages: 

• A single protocol for all input amounts 
• Fewer steps & less consumables waste 
• Fewer cleanups 
• Automation friendly

Figure 1: NEBNext UltraExpress™ DNA Library Prep workflow 

Figure 2: NEBNext UltraExpress™RNA Library Prep Kit workflow 

NEBNext UltraExpress™ DNA library prep kits

NEBNext UltraExpress is the next generation of NGS library prep, and the NEBNext UltraExpress DNA Library Prep Kit offers a one-size-fits-all protocol (within the stated input range) for simplified and streamlined library prep, from sample to high-quality library in under two hours! 

• Fast workflow (< 2 hours)  
• Fewer steps and consumables  
• Fewer cleanups  
• High-quality libraries from a wide input range (10 - 200 ng pre-sheared DNA)  
• Single protocol for all inputs 
• Automation friendly 

View or download extensive performance data in the Data Supplement.

NEBNext UltraExpress™ RNA library prep kits

The NEBNext UltraExpress RNA Library Prep Kit is the latest generation of NEBNext RNA library prep, and it has a fast, streamlined workflow. With a 3-hour library prep protocol, the kit enables creation of high-quality RNA libraries in a single day, in conjunction with mRNA enrichment or rRNA depletion kits.  

• Fast workflow (3 hours) 
• Fewer steps and consumables  
• Fewer cleanups  
• Single protocol for all input amounts 
• High-quality libraries from a broad input range: 25 - 250 ng total RNA 
• Automation friendly 

View or download extensive performance data in the  Data Supplement.