We offer cell culture media and reagents from Lonza and serum for cell growth from Biosera.
The range of products for cell culturing is enormous. We supply Greiner bio-one and TPP products, offering everything from standard cell culture products such as flasks, plates, tubes, cell scrapers and serological pipettes to specialized products such as 3D cell culture, vacuum filter system, cryoproducts for biobanking and plates for luminescence / fluorescence.
One of the most common contaminants in cell culture laboratories is mycoplasma. Cells are usually infected by cross-contamination from not tested infected cells (eg, through aerosols generated during pipetting, using the same medium bottles, working with more than one cell type at a time), contaminated material, contaminated donor tissue (<1%), or direct infection from the researcher.
Mycoplasma grows slowly and does not necessarily kill the cells, but affects different cellular parameters, such as changing the cell membrane's antigenicity, creating chromosomal level changes, inhibiting cell metabolism and fragmenting DNA.
To ensure accurate and reliable results from cell culture experiments, it is important to regularly test cell cultures for mycoplasma contamination. It can be challenging to detect mycoplasmas contamination because it does not cause change in pH or turbidity of the cell culture medium, which are common indicators of other contaminants.
We recommend MycoAlert ™ Mycoplasma Detection Kit from Lonza, a safe and easy test that takes very little time to perform.
We offer primary and secondary antibodies, controls etc. of the best quality from our suppliers: Cayman Chemical, Cell Signaling Technology (CST), GenScript, Origene, Rockland, and Vector Laboratories.
Feel free to contact us if you cannot find what you are looking for, we are happy to assist you.
We also offer antibody production if the antibody you need is not available commercially.
Chromatin immunoprecipitation (ChIP) is a powerful research technique used to identify and analyze protein-DNA interactions within the genome in vivo. It is a technique that aims to investigate whether specific proteins such as transcription factors and co-factor, replication factors and DNA repair proteins bind to specific genomic regions. Investigators can also determine specific targets of histone modifications in the genome.
Whatever your preferred method of chromatin preparation we have a ChIP kit suited to your needs.
Immunohistochemistry (IHC) is a technique commonly used for morphological characterization of tumors or other tissue malignancies. IHC uses antibodies to detect and analyze protein expression while maintaining the composition, cellular characteristics, and structure of native tissue. We offer IHC validated antibodies and detection systems.
Cayman's Prostaglandin E2 EIA kits are known worldwide, and the 8-Isopostane EIA Kit is often cited in oxidative stress studies, while the LDH Cytotoxicity Assay Kit is the most popular assay for cell viability.
Lonza offers specific assay kits for bone and fat cell types, as well as assays for cell proliferation, apoptosis and cytotoxicity that are very easy to use.